海岛棉愈伤组织诱导和分化研究

以海岛棉无菌苗的胚根、下胚轴和子叶作外植体，对这３种器官在离体培养中愈伤组织诱导、分化以及影响因素进行了研究。结果表明：下胚轴作外植体效果最好，愈伤组织生长块、质地好，有芽的分化，进而可再生出植株。胚根效果很差，愈伤组织产生很少而且很易褐化死亡。子叶效果不好，愈伤组织少、生长慢无分化。外源激素中２．４－Ｄ与ＫＴ配合使用效果好，愈伤组织可以分化。无菌苗生长状态及培养基中非激素成分对愈伤组织诱导和分化

The Study on the induction and Differentiation of Calli of Gossypium Barbadense.

Radicle.hypocotyl and cotyledon of Gossypium barbadense were used as explants. Theinduction differentiation of calli and the influential factors of these three organs under culturewere studied. As explant, hypocotyle was the best. its calli grew fast.differentiated bud andregenerated plant. Radicle was bad for explant. Cotyledon was not good for explant. its calliwere little and grew slowly. It was found that 2. 4-D plus KT was most effective on calli induction and the calli could differentiate. The growing status of the sterilized seeding and theno hormone factors in the medium had effect on calli induction and differentiation.