应用PCR技术检测结核分枝杆菌的临床分析

采用聚合酶链反应（Ｐｏｌｙｍｅｒａｎｓｅ Ｃｈｉａｎ Ｒｅａｔｉｏｎ，即ＰＣＲ）技术检测结核分枝杆菌Ｍｙｃｏｂａｃｔｅｒｉｕｍ ｔｕｂｅｒｃｕｌｏｓｉｓ，一年多来共检测了１００例结核（肺、肾结核）患者的痰和尿液标本，结果ＰＣＲ检出阳性率为８１％，对照用储菌涂片抗酸染色法，阳性率为５８％，用常规培养法阳性率为２０％。而对５０例非结核患者的痰和尿液标本的检测，ＰＣＲ法仍有６％的阳性率，而用涂片或常规培

Clinical analysis of the PCR for detection of Mycobacterium tuberculosis

Mycobacterium tuberculosis to the samples of 100 patients who take the tubercular were detected by used of the polymerase chain reaction(PCR),of which 81% were positive.But 58% of the patients were positive by the anti-acid staning mathod and 20% were positive by cultural mathod in ordinary medium.Addition,we used the PCR mathod for detecting Mycobacterium tuberculosis of samples from 50 non-tubercular patients,of which 6 % were also positive.However,the 50 samples were all negative by used of the cultural or the staining mathod.It is suggested that the PCR mathod be used as a rapid,sensitive and specific measure of detecting Mycobacterium tuberculosis.It is difficult to diagnose whether the patient takes pulmonary tuberculosis and kindey tuberculosis by the detective report,because some positive samples were detected in non-tubercular patients.