家兔胚胎冷冻保存——AgI植冰法的应用

以日本大耳白兔为供胚动物，采用ＦＳＨ－ｐ加ｈＣＧ超排。于交配后６２￣６５小时手术或屠宰，冲洗子宫角及输卵管采得正常的桑椹胚４０２枚。用两种植冰方法和两种装管顺序将兔胚置于塑料细管进行慢速冷冻。结果ＡｇＩ植冰法与传统的植冰法具有同样的植冰效果，其快速解冻后胚胎的可移植率分别为８５％和８０％；培养４８小时后发育至扩张囊胚的比例分别为９０％和８６％，均显著高于未植冰的两种装管方式（Ｐ〈０．０１）。ＡｇＩ

Cryopreservation of Rabbit Embryos-Utilization of Seeding Method by Silver Iodide(AgI)

The aim of present study was to develop an automatic and reliable seeding method byusing silver iodide(AgI) as the ice nucleator. The experiment was carried out on 402 morulastage rabbit embryos. Two methods of seeding and two methods of loading embryos intoplastic straws (total four groups) were used. The effectiveness of AgI was evaluated by invitro survival After thawing, the rate of trasferable embryos in four groups were 85, 10,80and 15% respectiviy, and the rate of embryos developed into expanded blastocysts were 90,13,86 and 18% respectiviy. The transferable and development rate of AgI seeding method(group A) was same as the conventional seeding method (group C), and higher than othergroups (B and D) without seeding. The seeding methed by AgI can be useful in the cryopreservation of rabbit embryos.