用中性红标记酵母原生质体初探

用２％蜗牛酶处理酵母细胞６０分钟，啤酒酵母Ｙ２９的原生质体形成率为９０％，再生率为９．５％；糖化酵母ＩＢ的原生质体形成率为８６％，再生率为１２％。用５００ｐｐｍ中性红染液对Ｙ２９菌株的整细胞和原生质体染色１５分钟，细胞的着色率为８４％，存活率为１２％，而原生质体的着色率为７５％，再生率为６．４％，经染色后的原生质体体积缩小，在交变电场中排队所需的场强电降低。

Preliminary Research on Marking Yeast Protoplast with Netural Red

The yeast protoplasts were made with common method, and they were detained with neturared. The results showed that after treating yeast cells with 2% snail enzyme for 60 min.Protoplast formation and regeneratioll frequency were 90% and 9. 554 respectively to S.cerevisiae Y29; 86% and 12% respectively to S. diastatics IB. After detaining Y29 cells andprotoplasts with 500ppm netural red for 15 min. Colored cell rate was 84%, survuved cell ratewas 12% ; Colored protoplast rate was 75%, regeneration rate was 6. 4%. The results alsoshowed that detaining can make cells shrinked, the line-up volatage lowed. In order to makefusion successful, authors ssuggested detained diploid or polyploid protoplasts fuse withundetained haploid protoplasts.