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大岩桐叶片培养再生植株的研究
引用本文:刘本叶,张艳萍.大岩桐叶片培养再生植株的研究[J].生物技术,1995,5(2):22-24.
作者姓名:刘本叶  张艳萍
作者单位:东北农业大学生物工程系
摘    要:以大岩桐(Sinningia speciosa Benth.et Hook)叶片为试材,接种于MS附加不同激素组合的培养基上,成功地经愈伤组织至球状体至不定芽途径形成大量再生植株并移栽成活。在各种细胞分裂素中,6-苄基嘌呤(BA)的效果优于激动素(KT)。生长素萘乙酸(NAA)与BA及KT配合使用时愈伤组织诱导、球状体分化及不定芽增殖均有明显的增效作用。其最适宜的培养基组成为:MS+BA2.0mg

关 键 词:大岩桐  愈伤组织  球状体  叶片培养  花卉

Studies on Plantlet Regeneration of Sinningia speciosa Benth. et Hook Through Leaf Culture
Liu Benye, Zhang Yanping, Zhang Xichun,Wu Jiangyun, Zhang Limei, Zhang Jianguong.Studies on Plantlet Regeneration of Sinningia speciosa Benth. et Hook Through Leaf Culture[J].Biotechnology,1995,5(2):22-24.
Authors:Liu Benye  Zhang Yanping  Zhang Xichun  Wu Jiangyun  Zhang Limei  Zhang Jianguong
Abstract:Leaves of Sinningia speciosa senth. et Hook were inoculated on MS solid media supplied with different combinations of phytohormone, and a large amount of plantlets were regenerated via. callus to globoids to adventitious bud and were successfully transplanted to soil.The effects of BA was superior to that of KT among cytokinins. Cullus induction, globoidsdifferentiation and adventitious bud multiplication were obviously promoted by the combina. non of BA or KT with NAA; The best medium was: MS BA 2. oppm NAA 0. Zppm, Onthis medium, the cullus induction rate was 94%, the globoid differentiation rate was 188%,the adventitious bud multiplication rate was 5 times at four week interval. The best rootingmedium was: MS NAA 0. bppm. The rooting rate was above 90%. The rate of alive transplanted plantlets was above 80%.
Keywords:Sinningia speciosa Benth  et Hook  Callus  Globoid  Adventitious bud  
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