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几种添加物对D—核糖产量的影响
引用本文:乔建军,杜连祥.几种添加物对D—核糖产量的影响[J].生物技术,2001,11(3):23-26.
作者姓名:乔建军  杜连祥
作者单位:天津轻工业学院食品工程系,
摘    要:研究了几种物质对D-核糖产量的影响,研究表明,当发酵培养基中玉米浆的含量为25g/L,D-核糖产量达到最高,为65g/L。发酵培养基中添加适量的粉及山梨醇亦有利于D-核糖的积累,当粉与山梨醇的添加量分别为10g/L及40g/L时,D-核糖产率分别增加7.8%、4.7%,而在发酵培养基中加入丙二酸可抑制D-核的分泌,在40ml发酵培养基中添加1ml丙二酸后,D-核糖的产率下降73.8%。甲醇也可抑制D-核糖的积累,当发酵培养基中的甲醇添加量为16g/L时,D-核糖产率下降66.2%。

关 键 词:D-核糖  短小芽胞杆菌  发酵法  产量  添加物
文章编号:1004-311X(2001)03-0023-04
修稿时间:2001年1月11日

Effects of several substances on production of D-ribose
Qiao Jian-jun,Du Lian-xiang.Effects of several substances on production of D-ribose[J].Biotechnology,2001,11(3):23-26.
Authors:Qiao Jian-jun  Du Lian-xiang
Abstract:Transketolase-deficient Bacillus pumilus stain was used to produce D-ribose in food and pharmacy industry.Effects of some substance on D-ribose production were researched in this paper.D-ribose production has been improved about 7.8% when 10g/L yeast powder was added in the medium,and D-ribose production has been improved about 4.7% when 10g/L sorbitol was added in the medium.The D-ribose titer reduced when the medium was added with other substance such as propane diacid.The fact that formic acid can reduce the D-ribose titer confirms the presence of the ribulose monophosphate pathway in Bacillus pumilus that has been founded in Bacillus subtilis.
Keywords:D-ribose  Bacillus subtilis  transketolase-defective  corm-steep liquor
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