| 罗布麻离体培养及快繁技术的研究 |
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| 引用本文： | 陈彦云,曹君迈,李国旗,孟军.罗布麻离体培养及快繁技术的研究[J].生物技术,2006,16(1):72-75. |
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| 作者姓名： | 陈彦云 曹君迈 李国旗 孟军 |
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| 作者单位： | 1. 宁夏大学生命科学学院,宁夏,银川,750021
2. 西北第二民族学院生命科学系,宁夏,银川,750021 |
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| 基金项目： | 国家林业局“948”项目(”罗布麻属耐盐植物新种及其培育技术引进,No.2004-4-10) |
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| 摘 要： | 采用野生罗布麻(Apocynum venetamL.)顶芽及芽下茎段为外植体进行离体培养及快繁技术优化的研究。结果表明:适宜罗布麻离体培养的基本培养基为MS,适宜外植体起始分化的培养基为:MS BA 1.8mg/L KT 0.5mg/L,分化率为88.9%以上;适宜茎段增殖的培养基为MS BA 2.0mg/L KT 0.5mg/L,繁殖系数最高达5.67倍,通过切段繁殖还可提高繁殖系数3倍;适宜生根的培养基为MS IBA 0.5mg/L NAA 0.02mg/L,生根率达90%以上。
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| 关 键 词： | 罗布麻 离体培养 快繁技术 |
| 文章编号： | 1004-310X（2006）01-0072-04 |
| 收稿时间： | 2005-07-15 |
| 修稿时间： | 2005-11-17 |
Research on Culture in vitro and Rapid Multiplication Techniques of Apocynum venetam |
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| CHEN Yan-yun,CAO Jun-mai,LI Guo-qi,MENG Jun.Research on Culture in vitro and Rapid Multiplication Techniques of Apocynum venetam[J].Biotechnology,2006,16(1):72-75. |
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| Authors: | CHEN Yan-yun CAO Jun-mai LI Guo-qi MENG Jun |
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| Institution: | 1. Ningxia University Life Sciences School, Yinchuan 750021 ; 2. Department of Life Science, the Second Northwest Institute for Ethnic Minorities, Yinchuan 750021, P. R. China |
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| Abstract: | The techniques of tissue culture and rapid propagation were researched with terminal buds and stem segments below bud of wild dogbanes(Apocynum venetam L.) as explants.The results showed that the basic medium suitable for culturing dogbanes(Apocynum venetam L.) in vitro was MS.The medium suitable for starting the differentiation of explants was MS BA 1.8mg/L KT 0.5mg/L with the differentiation rate of over 88.9%.The medium suitable for multiplication of stems was MS BA 2.0mg/L KT 0.5mg/L with the highest multiplication factor of(5.67) times,which could also increase 3 times by cutting stem.The medium suitable for rooting was MS IBA 0.5mg/L NAA 0.02mg/L with a rooting rate of over 90%. |
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| Keywords: | dogbane(Apocynum venetam) culture in vitro rapid propagation techniques |
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