| 一种有效的花瓣总RNA的提取方法 |
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| 引用本文： | 孟丽,周琳,张明姝,戴思兰.一种有效的花瓣总RNA的提取方法[J].生物技术,2006,16(1):38-40. |
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| 作者姓名： | 孟丽 周琳 张明姝 戴思兰 |
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| 作者单位： | 北京林业大学园林学院,北京,100083 |
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| 基金项目： | 国家林业局“948”项目(2001-24) |
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| 摘 要： | 利用CTAB法以富含花青素类物质的紫蓝色花瓣为材料提取总RNA,经紫外光谱分析A260/A280比值为1.9~2.0,A260/A230比值约为2.0;电泳检测到了28S、18S和5S rRNA清晰的条带;通过RT-PCR扩增出了目的基因的cDNA片段,说明分离的总RNA能去除色素干扰,纯度和反转录活性较高符合RNA相关实验的要求,是一种经济、有效的花瓣总RNA的提取方法。
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| 关 键 词： | 花瓣 RNA提取 CTAB RT-PCR |
| 文章编号： | 1004-311X（2006）01-0038-03 |
| 收稿时间： | 2005-09-21 |
| 修稿时间： | 2005-11-07 |
An Efficient and Economic Method for Preparation Total RNA of Petals |
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| MENG Li,ZHOU Lin,ZHANG Ming-shu,DAI Si-lan.An Efficient and Economic Method for Preparation Total RNA of Petals[J].Biotechnology,2006,16(1):38-40. |
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| Authors: | MENG Li ZHOU Lin ZHANG Ming-shu DAI Si-lan |
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| Institution: | College of Landscape Architecture, Beijing Forestry University, Beljing 100083, China |
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| Abstract: | Total RNA was isolated fnam indigo petals with CTAB method. UV - Spectroscopic analysis showed that A260/A280 = 1.9 - 2.0 and A260/ A230 = 2.0; 28S, 18S and 5S rRNA were all clear in electrophoresis picture; Target gene was amplified from isolated total RNA by RT- PCR, which testified high quality RNA could be isolated from petals with this method. |
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| Keywords: | petals RNA isolation CTAB RT-PCR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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