| 陆地棉EST长度多态性与其SSR分布特征相关性分析 |
| |
| 引用本文： | 王亚男,陈允峰,樊洪泓,李廷春,林毅,蔡永萍.陆地棉EST长度多态性与其SSR分布特征相关性分析[J].生物技术,2010,20(1):1-4. |
| |
| 作者姓名： | 王亚男 陈允峰 樊洪泓 李廷春 林毅 蔡永萍 |
| |
| 作者单位： | 安徽农业大学生命科学学院,安徽,合肥,230036 |
| |
| 基金项目： | 安徽省"十一五"科技攻关项目，安徽省教育厅重点项目，高校博士点项目 |
| |
| 摘 要： | 目的:分析陆地棉EST长度多态性与其SSR分布特征的相关性。方法:从NCBI公共数据库下载陆地棉EST序列,应用SSRIT搜索SSR,分析20 000条无冗余的EST序列。结果:在剔除低质量和冗余的序列后,得到全长为7 363.878kb的无冗余EST序列7 322条,其中含有SSR位点的EST序列数520条,占被分析EST比例的2.60%。长度在400bp以下的EST序列含SSR的比例为1.46%;长度在400bp以上的EST序列含SSR的比例为8.94%。在1～6bp的重复基元中,二核苷酸重复基元的SSR重复频率最高,占总数的63.46%,其次是三核苷酸,占总数的34.04%。二核苷酸类型(AG)n、(AT)n和三核苷酸类型(AAG)n、(ACC)n、(ACT)n、(AAT)n是SSR的主要重复基元。结论:棉花EST-SSR可用于棉花分子标记,为有针对性设计陆地棉EST-SSR引物奠定基础。
|
| 关 键 词： | 陆地棉 EST—SSR 序列长度 重复基元 |
Relationship Analysis between Length of ESTs and Characters of EST-SSR for Gossypium hirsutum |
| |
| WANG Ya-nan,CHEN Yun-feng,FAN Hong-hong,LI Ting-chun,LIN Yi,CAI Yong-ping.Relationship Analysis between Length of ESTs and Characters of EST-SSR for Gossypium hirsutum[J].Biotechnology,2010,20(1):1-4. |
| |
| Authors: | WANG Ya-nan CHEN Yun-feng FAN Hong-hong LI Ting-chun LIN Yi CAI Yong-ping |
| |
| Institution: | WANG Ya-nan,CHEN Yun-feng,FAN Hong-hong,LI Ting-chun,LIN Yi,CAI Yong-ping(College of Life Sciences,Anhui Agricultural University,Heifei 230036,China) |
| |
| Abstract: | Objective: The relationship between length of Gossypium hirsutum ESTs sequence and characters of EST-SSR was researched.Method:In this study,the 20 000 ESTs of Gossypium hirsutum which had no redundancy sequences were downloaded from NCBI,and SSRs were screened by using SSRIT program.20 000 EST sequences were analyzed.Result: 7 322 unique sequences with total length 7 363.878kb were obtained after the removement of the ESTs with redundant sequences and low qualities.Gossypium hirsutum had 520 EST sequences ... |
| |
| Keywords: | EST-SSR Gossypium hirsutum EST-SSR sequence length repeat motif |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
