| 草麻黄植株再生体系的建立 |
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| 引用本文： | 张红梅,蔡宝宏,王明艳.草麻黄植株再生体系的建立[J].生物技术,2010,20(2):72-74. |
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| 作者姓名： | 张红梅 蔡宝宏 王明艳 |
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| 作者单位： | 1. 华北煤炭医学院生物科学系河北唐山063000
2. 唐山市第一中学生物组,河北唐山,063000 |
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| 基金项目： | 河北省教育厅项目，河北省唐山市科技项目 |
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| 摘 要： | 目的:以草麻黄的子叶为材料,建立草麻黄植株再生体系.方法:采用组织培养的方法进行了愈伤组织诱导、愈伤组织分化和不定芽生根研究.结果:MS+2,4-D 2.0mg/1+6-BA 1.0mg/l为诱导子叶形成具有分化能力愈伤组织的理想培养基;MS+2,4-D 1.5mg/l+6-BA 1.5mg/l是愈伤组织的最佳继代培养基;MS+IAA 0.2mg/l+TDZ 2.0mg/l是愈伤组织不定芽分化的最佳培养基,分化率为75%;试管苗生根培养基为MS+2,4-D 1.0mg/l.结论:建立了草麻黄子叶再生系统,为开发和保护麻黄野生资源提供一定的材料来源和技术方法.
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| 关 键 词： | 草麻黄 组织培养 愈伤组织 植株再生 |
Establishment of Regeneration System of Ephedrae sinica Stapf |
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| ZHANG Hong-mei,CAI Bao-hong,WANG Ming-yan.Establishment of Regeneration System of Ephedrae sinica Stapf[J].Biotechnology,2010,20(2):72-74. |
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| Authors: | ZHANG Hong-mei CAI Bao-hong WANG Ming-yan |
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| Institution: | ZHANG Hong-mei1,CAI Bao-hong2,WANG Ming-yan1(1.Department of Biological Science,North China Coal Medical College,Tangshan,Hebei 063000,China,2.The First Middle School of Tangshan,Tangshan 063000,China) |
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| Abstract: | Objective:To establish the regeneration system of Ephedrae sinica Stapf cotyledon.Method:The conditions needed in callus induction,differentiation and adventitious bud rooting of Ephedrae sinica Stapf cotyledon have been studied.Result:The results showed that the best medium for callus induction was MS+2,4-D 2.0mg/l+6-BA 1.0mg/l with ability of differentiation.The best media of callus generation was MS+2,4-D 1.5mg/l+6-BA 1.5mg/l.The best media of differentiation was MS+IAA 0.2mg/l+TDZ 2.0mg/l,differentiatio... |
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| Keywords: | Ephedrae sinica Stapf tissue culture callus plantlet genetration |
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