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| CBF4基因植物表达双元载体的构建 | |
| 引用本文： | 沙丽娜,郭兆奎,万秀清,颜培强,刘丹,刘磊.CBF4基因植物表达双元载体的构建[J].生物技术,2009,19(4):6-9. |
| 作者姓名： | 沙丽娜 郭兆奎 万秀清 颜培强 刘丹 刘磊 |
| 作者单位： | 1. 黑龙江八一农垦大学植物科技学院,黑龙江,大庆,163319 2. 黑龙江八一农垦大学植物科技学院,黑龙江,大庆,163319;黑龙江省烟草科学研究所,黑龙江,牡丹江,157011 3. 黑龙江省烟草科学研究所,黑龙江,牡丹江,157011 |
| 基金项目： | 国家烟草专卖局科技计划项目 |
| 摘 要： | 目的:对拟南芥CBF4基因序列进行克隆.方法:用限制性内切酶将CBF4基因从pMD18-T CBF4载体上切下,定向连接到含超强启动子的pC2301-35S-OCS表达载体上,成功构建了CBF4基因植物表达载体pC2301-35S-OCS-CBF4.利用冻融法将此表达载体导入只含辅助质粒的根癌农杆菌中,提取转化质粒,经PCR扩增和酶切验证鉴定表明.结果:CBF4基因植物表达双元载体构建成功.结论:转CBF4基因烟草的抗寒性比野生型烟草要高. |
| 关 键 词： | CBF4基因 植物表达载体 抗寒性 |
Construction of CBF4 Gene in Plant Expression Binary Vector |
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| SHA Lina,GUO Zhao-kui,WAN Xiu-qing,YAN Pei-qiang,LIU Dan,LIU Lei.Construction of CBF4 Gene in Plant Expression Binary Vector[J].Biotechnology,2009,19(4):6-9. | |
| Authors: | SHA Lina GUO Zhao-kui WAN Xiu-qing YAN Pei-qiang LIU Dan LIU Lei |
| Institution: | SHA Li-na1,GUO Zhao-kui1,2*,WAN Xiu-qing2,YAN Pei-qiang2,LIU Dan1,LIU Lei1(1.Heilongjiang August First Land Reclamation University,Daqing 163000,2.Heilongjiang Tobacco Research Institute,Mudanjiang 157011,China) |
| Abstract: | Objective:CBF4 gene was cloned and then was cut from pMD18-T-CBF4 vector with restraction enzymes.Method:The fragment of CBF4 gene in pC2301-35s-OCS was directively ligated,The plant expression vector pC2301-35s-OCS CBF4 was constructed.The plant expression vector was transformed into Agrobacterium tumefaciens which only contained assistant plasmid in order to construct double-plasmid expression vector.The binary expression vector plasmid was identified by PCR reaction and restriction endonuclease digestion... |
| Keywords: | CBF4 gene plant expression vector cold tolerance |
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