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| GNA成熟蛋白基因亚克隆及其原核表达载体构建 | |
| 引用本文： | 罗素兰,林俊芳,陈如凯,唐克轩,郭丽琼.GNA成熟蛋白基因亚克隆及其原核表达载体构建[J].生物技术,2002,12(6):1-2. |
| 作者姓名： | 罗素兰 林俊芳 陈如凯 唐克轩 郭丽琼 |
| 作者单位： | 1. 海南大学海洋学院,海南,海口,570228;热带作物生物技术国家重点实验室,海南,海口,571101 2. 热带作物生物技术国家重点实验室,海南,海口,571101 3. 福建农林大学甘蔗综合研究所,福建,福州,350002 4. 复旦大学遗传工程国家重点实验室,上海,200433 |
| 基金项目： | 海南省自然科学基金资助项目 ( 30 1 1 1 ) |
| 摘 要： | 雪花莲外源凝集素（GNA）对刺吸式昆虫和某些咀嚼式昆虫以及多种线虫均有毒性，从含GNA前体蛋白基因的质粒中亚克隆出GNA的成熟蛋白基因MGNA，将MGNA基因插入大肠杆菌表达载体pET22b的不同位点，再经测序验证，得到了三种不同表达形式的GNA原核表达载体；22bG1(分泌型融合GNA蛋白），22bG2(包涵体型GNA蛋白），22bG3(分泌型天然GNA蛋白），这为进一步在大肠杆菌中表达GNA和将GNA制成生物农药奠定了基础。 |
| 关 键 词： | 雪花莲外源凝集素基因 大肠杆菌 GNA成熟蛋白基因 亚克隆 原核表达载体 |
| 文章编号： | 1004-311X(2002)06-0001-02 |
| 修稿时间： | 2002年7月8日 |
Subcloning of GNA mature protein (MGNA) gene and its construction of expression vector in E.coli |
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| LUO Su-Lan ,LIN Jun-Fang,CHEN Ru-Kai,TANG Ke-Xuan,GUO Li-Qiong .College of Oceanography,Hainan University,Haikou ,.National Key Biotechnology Laboratory for Tropical Crops,Haikou.Subcloning of GNA mature protein (MGNA) gene and its construction of expression vector in E.coli[J].Biotechnology,2002,12(6):1-2. | |
| Authors: | LUO Su-Lan LIN Jun-Fang CHEN Ru-Kai TANG Ke-Xuan GUO Li-Qiong College of Oceanography Hainan University Haikou National Key Biotechnology Laboratory for Tropical Crops Haikou |
| Institution: | LUO Su-Lan 1,2,LIN Jun-Fang2,CHEN Ru-Kai3,TANG Ke-Xuan4,GUO Li-Qiong2 1.College of Oceanography,Hainan University,Haikou 570228,2.National Key Biotechnology Laboratory for Tropical Crops,Haikou 571101,3.Institute of Sugarcane,Fujian Agriculture and Forestry University,Fuzhou 350002,4.State Key Laboratory of Genetic Engineering,Fudan University,Shanghai 200433) |
| Abstract: | Snowdrop lectin mature protein is toxic to sap sucking insects and some chewing pests and many kinds of nematode.Mature protein gene of snowdrop lectin (MGNA) was subcloned in T vector from plasmid containing its precursor protein gene.MGNA was inserted downstream of T7 promoter at three different sites of E.coli expression vector pET22b(+),resulting in the construction of three prokaryotic expression vectors p22bG1(secretion of fusion GNA),p22bG2(Inclusion body) and p22bG3 (secretion of native GNA). The three vectors give different types of MGNA product expressed in E.coli. This research will be the base for MGNA expression in E.coli and make it into bio-pesticide. |
| Keywords: | snowdrop lectin (Galanthus nivalis agglutinin) mature protein gene prokaryotic expression vectors construction |
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