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一株AEC^hrL—赖氨酸产生菌
引用本文:刘复军.一株AEC^hrL—赖氨酸产生菌[J].生物技术,1993,3(5):25-29,F003.
作者姓名:刘复军
作者单位:黑龙江省轻工业研究所 哈尔滨
摘    要:从乳糖发酵短杆菌Brevibacterium Lactofermentum2645菌株出发选育L-赖氨酸产生菌,经分离复壮后,用硫酸二乙酯(DES)诱变处理,由添加S-(2-氨基乙基)—L—半胱氨酸(AEC)和L-苏氨酸的药物平板定向筛选,最后获得了一株L—赖氨酸高产菌E_(16-2)(AHV~(hr)SAM~gAEC~(hr))。进而通过正交试验、工艺条件试验最终获得了该菌株的最佳发酵条件,并考查了在此条件下的L-赖氨酸发酵过程,结果表明该菌具有生长、耗糖、产酸速度快、发酵周期短的明显特点。

关 键 词:赖氨酸  赖氨酸产生菌  诱发变异

A strain of L-Lysine Producing with AKC~(hu)
Liu Fujun.A strain of L-Lysine Producing with AKC~(hu)[J].Biotechnology,1993,3(5):25-29,F003.
Authors:Liu Fujun
Institution:Light Industry Institute of HeiLongjang Province Harbin
Abstract:Selected the L-Lysine producing strain from Brevibacterium Lactoferm -entum 2645. After isolated and resurgeneed, a high producing L -Lysine mutant strain E16-2 (AHVhrSAMg AEChr) was abtaiied, mutagenized with DES isolated from the medicine plate which added L-Threoniue and AEC.Then the optimun culture conditions was found by the tests of orthogonal desing and technological conditions.The result of experiment show that the mutant strain E16-2 is of the chara-cters with fast growing, fast exhausting sugar, fast producing L-Lysine and short fermentation period.
Keywords:L- Lysine  B  lactofermeatum  AEC  Mutagonize
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