| 耐碱性木聚糖酶产酶菌株的选育 |
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| 引用本文： | 问清江,吴小杰,李叶昕,慕娟,党永.耐碱性木聚糖酶产酶菌株的选育[J].生物技术,2010,20(1):33-36. |
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| 作者姓名： | 问清江 吴小杰 李叶昕 慕娟 党永 |
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| 作者单位： | 陕西省微生物研究所,陕西,西安,710043 |
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| 基金项目： | 陕西省科学院社会可持续发展专项 |
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| 摘 要： | 目的:筛选出产碱性木聚糖酶酶活力高的菌株。方法:从碱性环境中采集样品,以自制木聚糖为惟一利碳源,采用刚果红透明圈法于摇瓶发酵法相结合筛选出18株产木聚糖酶酶活较高的菌株,其中1株酶活最高可达335.14 IU/ml。结果:通过培养特性及形态特征初步鉴定为芽孢杆菌。对部分酶学性质研究的结果表明:其作用最适温度60℃,最适pH8.0,在pH9.0条件下仍具有80%酶活力。结论:属于耐碱性木聚糖酶,具有很好的工业应用前景。
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| 关 键 词： | 碱性 木聚糖酶 芽孢杆菌 筛选 |
Screening of the Alkali Resistance Xylanase Producing Strains |
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| WEN Qing-jiang,WU Xiao-jie,LI Ye-xin,Mu Juan,DANG Yong.Screening of the Alkali Resistance Xylanase Producing Strains[J].Biotechnology,2010,20(1):33-36. |
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| Authors: | WEN Qing-jiang WU Xiao-jie LI Ye-xin Mu Juan DANG Yong |
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| Institution: | WEN Qing-jiang,WU Xiao-jie,LI Ye-xin,MU Juan,DANG Yong(Shanxi Institute of Microbiology,Xi\'an 710043,China) |
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| Abstract: | Objective: Screening produces alkaline xylanase enzyme activity of high strain.Method:18 xylanase producing strains were obtained from alkaline condition by assaying congo-red hydrolyzed circle on the selective medium,and xylanase activity under shake-flask fermentation,in which xylan were used as the only carbon source,and the maximum enzyme activity achieved 335.14 IU/ml.It was tentatively identified as Bacillus based on its morphological characterization.Result:The study on characteristics of xylanase wa... |
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| Keywords: | alkaline xylanase Bacillus screening |
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