TRAP及SSCP检测草鱼微卫星序列多态性

目的：在草鱼不同种群遗传结构比较中，应用一些来自鲤鱼的微卫星引物扩增草鱼的相关序列缺少多态性，该研究通过应用几种方法提高这些序列在遗传学研究中的价值。方法：运用TRAP随机引物与鲤鱼的微卫星引物组合，扩增出多态性好、重复性强的产物；同时采用SSCP技术对那些没有长度多态的微卫星序列进一步分析，寻找这些序列存在一些核苷酸位点的多态性。结果：TRAP可以检查到多态性好、重复性强的产物；SSCP发现一部分没有长度多态性的微卫星序列存在核苷酸位点多态性并且可以清楚分辨不同的基因型。结论：结果表明TRAP及SSCP在种群遗传学研究有良好的应用前景。

Detection of Polymorphism in Microsatellite Sequences by TRAP and SSCP

Objective:The primers originated from common carp are applied into population genetics of grass carp.But no polymorphism in length is detected.Some methods are applied to improve the useness of these primers.Methods:The arbitrary primers of TRAP and microsatellite primers are combined for amplification of the flank sequence of microsatellite sequences of Ctenopharyngodon idella and SSCP has also been applied for further analysis of these microsatellite sequences,Result:Relatively high polymorphism in length is detected by combination of arbitary primers from TRAP and microsatellite primers.Some changes are also found in nucleic acid sequences and different gene types can also been distinguished by SSCP.Condusion:TRAP and SSCP is a useful method in research work of population genetics.Some microsatellite sequences without polymorphism in length are also used in population genetics.