一株纤维素分解菌的分离与筛选

以新华滤纸为唯一碳源，从垃圾堆肥中筛选能够分解纤维素的菌株共39株，采用刚果红鉴别培养基进行识别，获取透明圈较大的菌株10株，在此基础上，进行液体培养，测定酶活，得到1株酶活较高的曲霉B－6（Aspergillus sp)。将B－6与绿色木霉（Trichoderma sp)AS3.3711进行了参比试验，比对筛选工作进行评定，经过固体，液体发酵对比试验，发现B－6与AS3．371有相近的产酶性能，B－6在固，液发酵中酶活分别达到39．2IU，14．9IU，而S3．3711则分别为16．6IU与15．7IU，且B－6较AS3．3711有更强的液化CMC的能力，B－6在24h内即能使3％CMC完全液化，而S3．3711则需96h。

Isolation and screening of cellulose-decomposing microorganisms

The 39 strains of cellulose-decomposing microorganisms were isolated from rubbish compost by Dubos liquid medium with Xinhua filter paper as the sole carbon source.We got 10 colonies surrounded by some distinct zones of clearing in cellulose-congo red agar medium from the 39 strains above.By measuring the enzyme activity of liquid culture filtrates,we got a strain(termed B-6)with higher cellulase activity.In order to evaluate our screening,the results of liquid and solid fermentation were compared between strain B-6 and AS33 711.We found out that they had similar enzyme-producing property.The enzyme activity of B-6 reached 39.2 and 14.9units,comparied to AS3.3711 with 16.6 and 15.7 units in solid and liquid fermentation respectively.In addition,B-6 had higher capacity of liquefying CMC.It could liquidize 3% CMC completely within 24 hours,while AS3.3711 needed 96h to do it.