| 原生质体融合子代的筛选和鉴定 |
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| 引用本文： | 邱雁临,曾莹,胡赛阳,夏服宝.原生质体融合子代的筛选和鉴定[J].生物技术,2003,13(6):17-19. |
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| 作者姓名： | 邱雁临 曾莹 胡赛阳 夏服宝 |
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| 作者单位： | 湖北工学院生物工程系,湖北,武汉,430068 |
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| 基金项目： | 湖北省科技厅重点攻关项目 (编号 :2 0 0 1AA2 0 5B0 9) |
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| 摘 要： | 介绍了绿色木霉N6和黑曲霉856原生质体融合子代的研究。经传代、发酵、筛选，从11株初筛的融合子中得到了3株纤维素酶活高且稳定的菌株AT23、AT16、AT34，其CMC酶活分别为亲本绿色木霉N6的2．2倍、1．4倍、1．2倍。并对其进行制霉菌素抗性试验和可溶性蛋白质凝胶电泳分析鉴定。试验结果证明了AT23、AT16、AT34是基因发生了重组的融合子且具有杂种优势。
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| 关 键 词： | 原生质体 融合子 传代 发酵 筛选 |
| 文章编号： | 1004-311X(2003)06-0017-03 |
| 修稿时间： | 2003年6月2日 |
Screening and Identifying of Protoplasts Fusion Progeny |
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| QIU Yan-lin,ZENG Ying,HU Sai-yang,XIA Fu-bao.Screening and Identifying of Protoplasts Fusion Progeny[J].Biotechnology,2003,13(6):17-19. |
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| Authors: | QIU Yan-lin ZENG Ying HU Sai-yang XIA Fu-bao |
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| Abstract: | This paper introduced the study of protoplast fusion progeny betwee Trichoderma viride N_6 and Aspergillus niger 856.By generating,fermenting and screening 3 strains AT23,AT16,AT34 with high cellulase activity and stalle were abtained from 11 strains primary screening fusant.The value of their CMC ase activity is 2.2,1.4,1.2 times respectively of that of N_6.By anti-nystain ability testing and souluable protens SDS-PAGE analyzing identity.The results proved that the 3 strains are gene combination fusion progeny possessing hybridization domination. |
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| Keywords: | Fusant Generating Ferment Screening Identify |
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