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| 大肠杆菌不耐热肠毒素B亚单位的克隆、表达及初步纯化 | |
| 引用本文： | 黄思扬,马超,雷清,蒋琳,沈心亮,王锐.大肠杆菌不耐热肠毒素B亚单位的克隆、表达及初步纯化[J].生物技术,2005,15(6):17-20. |
| 作者姓名： | 黄思扬 马超 雷清 蒋琳 沈心亮 王锐 |
| 作者单位： | 1. 兰州大学生命科学学院,甘肃,兰州,730000 2. 兰州生物制品研究所,甘肃,兰州,730046 3. 北京生物制品研究所,北京,100024 |
| 基金项目： | 甘肃省自然科学基金（No.3ZS051-A25-094） |
| 摘 要： | 为研究大肠杆菌不耐热肠毒素B亚单位（LTB）的佐剂活性。从大肠杆菌中调出LTB的原始基因，将该基因克隆、构建pET21b—LTB表达载体、转化大肠杆菌B121（DE3）进行表达，并对表达产物进行初步纯化；经DNA测序、SDS—PAGE、ELISA检测，结果表明成功构建了能够稳定表达可溶性LTB的菌株，并获得初步纯化LTB的方法。为今后LTB的研究及应用奠定了基础。 |
| 关 键 词： | 大肠杆菌不耐热肠毒素B亚单位 表达 纯化 粘膜佐剂 |
| 文章编号： | 1004-311X（2005）06-0017-04 |
| 收稿时间： | 2005-08-29 |
| 修稿时间： | 2005-09-15 |
Cloning, Expression and Purification of Escherichia coli Heat- labile Enterotoxin B subuint |
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| HUANG Si-yang,MA Chao,LEI Qing,JIANG Lin,SHEN Xin-liang,WANG Rui.Cloning, Expression and Purification of Escherichia coli Heat- labile Enterotoxin B subuint[J].Biotechnology,2005,15(6):17-20. | |
| Authors: | HUANG Si-yang MA Chao LEI Qing JIANG Lin SHEN Xin-liang WANG Rui |
| Institution: | 1. School of Life Science, Lanzhou University, Lanzhou,730000, China; 2. Beijing Institute of Biological Products, Beijing, 100024, China;3.Lanzhou Institute of Biological Products, Lanzhou, 730046, China |
| Abstract: | In order to achieve the expression of LTB in E.coli BL21(DE3) and to study its adjuvant activity.LTB gene was amplified by PCR,and pET-21b-LTB expression vector was constructed,then it was transformed in E.coli BL21(DE3).It was detected by sequencing DNA,SDS-PAGE and ELISA.Result:the expression of LTB was constructed successfully,the way for purify LTB was obtain. |
| Keywords: | Escherichia coli heat -iabile enterotoxin B subuint expression purification mucosal immunoadjuvant |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |