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假单胞菌S-2降解甲胺磷性能的研究
引用本文:武晓炜,吴志国,王艳敏,董超,张丽萍,史延茂,杨迎霞.假单胞菌S-2降解甲胺磷性能的研究[J].生物技术,2005,15(1):77-79.
作者姓名:武晓炜  吴志国  王艳敏  董超  张丽萍  史延茂  杨迎霞
作者单位:1. 河北工业大学研究生学院生物工程系,天津,300130
2. 河北省科学院生物所,河北,石家庄,050051
3. 石家庄市栾城县教育局,河北,石家庄,050000
基金项目:河北省科技厅资助项目 (0 1 2 2 0 1 36D)
摘    要:从甲胺磷生产车间分离到一株假单胞菌编号为S-2。S-2可利用甲胺磷为唯一氮源,但不能利用甲胺磷为唯一磷源。该文对S-2体内具有的降解甲胺磷的酶类进行了研究,初步断定:S-2可代谢产生酸性磷酸酶,主要在胞外降解甲胺磷。S-2在甲胺磷诱导的情况下,这些降解酶类可大量聚积。用诱导过的菌液降解甲胺磷比未经诱导的快了2d左右。

关 键 词:甲胺磷  降解    假单胞菌

Study on Degradation of Methamidophos by Pseudomonas sp. S-2
WU Xiao-wei,WU Zhi-guo,WANG Yan-min,DONG Chao,ZHANG Li-ping,SHI Yan-Mao,YANG Yin-xia.Study on Degradation of Methamidophos by Pseudomonas sp. S-2[J].Biotechnology,2005,15(1):77-79.
Authors:WU Xiao-wei  WU Zhi-guo  WANG Yan-min  DONG Chao  ZHANG Li-ping  SHI Yan-Mao  YANG Yin-xia
Institution:WU Xiao-wei1,WU Zhi-guo1,WANG Yan-min1,DONG Chao2,ZHANG Li-ping2,SHI Yan-mao
Abstract:Pseudomonas sp.S-2 is separated from the methamidophos workroom.S-2 oneself can make use of methamidophos as the unique source of N,but it can't make use of methamidophos as the unique source of P.It is concluded that S-2 has enzyme which can degradate methamidophos,and that S-2 can be produced by metabolism acidity phosphatase,and the degradation of methamidophos mainly happens outside the cell.Under the situation of the methamidophos induce,these degradation enzyme separated from S-2 can accumulate in large number.When the germ is induced,the velocity of degradation methamidophos is two days earlier than the contraposition which are not induced.
Keywords:methamidophos  degradate  enzyme  Pseudomonas sp  
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