| 产壳聚糖酶菌株的发酵条件优化 |
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| 引用本文： | 贾艳萍,张兰河,韩利.产壳聚糖酶菌株的发酵条件优化[J].生物技术,2010,20(2):62-64. |
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| 作者姓名： | 贾艳萍 张兰河 韩利 |
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| 作者单位： | 东北电力大学化学工程学院,吉林吉林,132012 |
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| 摘 要： | 方法:通过单因子实验,对保存的1株产壳聚糖酶的菌株C001进行发酵产酶条件优化,确定了最适产酶培养基组分.结果:温度30℃,发酵时间18h,pH5.5,接种量5%优化发酵条件后,产壳聚糖酶活力增长了37.4%.
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| 关 键 词： | 壳聚糖酶 发酵条件优化 酶活力 |
Study on Optimal Fermentation Conditions of a Chitosanase-producing Bacterium |
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| JIA Yan-ping,ZHANG Lan-he,HAN Li.Study on Optimal Fermentation Conditions of a Chitosanase-producing Bacterium[J].Biotechnology,2010,20(2):62-64. |
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| Authors: | JIA Yan-ping ZHANG Lan-he HAN Li |
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| Institution: | JIA Yan-ping,ZHANG Lan-he,HAN Li(Department of Chemistry,Northeast China College of Electric Power Engineering,Jilin 132012,China) |
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| Abstract: | Objective:A swain named C001 with ehitosanased activity was isolated from soil samples bymono- factor method .The obtain-merit of optimal compositions of the medium was our objective.Result: The optimal fermentation eondions for COOl produeing ehitosanase were as follows :cultivating temperature 30℃, fermentation time 18h,Ph5.5,5％ (V/V)amount of inoculation.The conclusion of the chitosanase activity of fermented broth by optimal fermentation can be increased by 37.4％. |
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| Keywords: | chitosanase optimal fermentation conditions chitosanase activity |
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