喷雾:一种简便的拟南芥转化方法

目的：建立一种改良的且操作更为简便可行的拟南芥转化方法。方法：用含有OD600等于0．8的农杆菌,5%蔗糖和0. 2 ml/L表面活性剂Silwet L-77的MS液体培养基喷洒正在发育的花器官即可实现基因转化。植物生长到高约4cm时进行转化,此时植物具有大量的花和极少数的果荚,可以得到比较高的转化效率。喷洒溶液后将植物用保鲜膜等材料包裹起来以保持湿度,可有利于转化。结果：得到了365棵转化植株,转化效率和花器官浸泡法的基本一致。结论：这种方法可适用于多种生态型的拟南芥植物转化,并且方便大规模的拟南芥植物的转化,便于人们快速获得带有T-DNA标记的植株或基因遗传互补工作的顺利开展。

Spraying:A Simplified Method for Transformation of Arabidopsis

Objective:To establish a modified and simplified transformation method for Arabidopsis thaliana.Methods:In this method,an MS liq- uid culture medium containing Agrobacterium whose OD_(600) value was 0.8,0.5% sucrose and 0.2ml/L surfactant Silwet L-77 was used to spray developing flowers.Plants inoculated when numerous immature floral buds and few siliques were present produced transformed progeny at a rela- tively higher rate.Covering plants for two to three days to retain humidity after inoculation was conducive to transformation.Results:With this method 365 transformants were obtained.The transformation rate was almost the same as that of floral dip method.Conclusion:Multiple ecotypes were transformable by this method.The modified method should facilitate high-throughput transformation of Arabidopsis for efforts such as T- DNA gene tagging or attempts at targeted gene replacement.