| 一株拮抗可可球二孢菌放线菌的分离及鉴定 |
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| 引用本文： | 卞光凯,缪倩,秦盛,陈永强,曹成亮,王祺琦,陈凤美,蒋继宏.一株拮抗可可球二孢菌放线菌的分离及鉴定[J].生物技术,2011,21(4). |
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| 作者姓名： | 卞光凯 缪倩 秦盛 陈永强 曹成亮 王祺琦 陈凤美 蒋继宏 |
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| 作者单位： | 徐州师范大学江苏省药用植物生物技术重点实验室,江苏徐州,221116 |
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| 基金项目： | 国家自然科学基金项目，"十一五"科技支撑项目 |
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| 摘 要： | 目的:筛选具有拮抗植物病原真菌可可球二孢菌活性的放线菌.方法:选择高氏一号培养基分离放线菌,以可可球二孢菌为指示菌进行抑菌活性筛选,通过形态学特征、生理生化特征、培养特征以及16S rRNA基因序列分析等研究对筛选得到的高活性菌株进行菌种鉴定.结果:从南方红豆杉根际土壤中筛选得到一株高活性菌株KLBMP 2284,16S rRNA基因序列比对结果显示其与弗吉尼亚链霉菌(Streptomyces virginiae)相似性98.963％,发酵液稀释300倍后抑制率为41.64％.结论:KLBMP 2284为弗吉尼亚链霉菌的一个菌株.
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| 关 键 词： | 放线菌 可可球二孢菌 抑菌活性 鉴定 16S rRNA基因 |
Isolation and Identification of One Actinomycete Strain Exhibiting Antifungal Activity against Lasiodiplodia theobromae |
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| BIAN Guang-kai,MIAO Qian,QIN Sheng,CHEN Yong-qiang,CAO Cheng-liang,WANG Qi-qi,CHEN Feng-mei,JIANG Ji-hong.Isolation and Identification of One Actinomycete Strain Exhibiting Antifungal Activity against Lasiodiplodia theobromae[J].Biotechnology,2011,21(4). |
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| Authors: | BIAN Guang-kai MIAO Qian QIN Sheng CHEN Yong-qiang CAO Cheng-liang WANG Qi-qi CHEN Feng-mei JIANG Ji-hong |
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| Institution: | BIAN Guang-kai,MIAO Qian,QIN Sheng,CHEN Yong-qiang,CAO Cheng-liang,WANG Qi-qi,CHEN Feng-mei,JIANG Ji-hong*(Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province,Xuzhou Normal University,Xuzhou 221116,China) |
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| Abstract: | Objective:To screen actinomycetes with anti-pathogenic fungi activity against Lasiodiplodia theobromae.Method:Actinomycetes were isolated by Gause I agar medium,then L.theobromae was selected to screen for the activity of anti-pathogenic fungi.The strain with high bioactivity was identified by the morphological,physiological,cultural characteristics and 16S rRNA gene sequence similarity analysis.Result:Strain KLBMP 2284 with high bioactivity was isolated from the rhizospheric soil of Taxus chinensis,similar... |
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| Keywords: | actinomycetes Lasiodiplodia theobromae antimicrobial activity identification 16S rRNA gene |
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