| 赭曲霉的高密度培养对坎利酮转化的影响 |
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| 引用本文： | 刘晓,刘逸寒,别松涛,徐星,谢进霞,李建勋,韩开林,路福平.赭曲霉的高密度培养对坎利酮转化的影响[J].生物技术,2011,21(5). |
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| 作者姓名： | 刘晓 刘逸寒 别松涛 徐星 谢进霞 李建勋 韩开林 路福平 |
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| 作者单位： | 工业发酵微生物教育部重点实验室,工业酶国家工程实验室,天津市工业微生物重点实验室,天津科技大学生物工程学院,天津300457 |
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| 基金项目： | 国家“863”项目，天津市科技支撑计划重点项目 |
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| 摘 要： | 目的:研究赭曲霉高密度培养的发酵培养基及条件,实现坎利酮的高转化.方法:选取廉价易得的培养基成分并进行优化,同时对发酵条件进行优化,得到了最优发酵培养基配方及培养条件.结果:发酵培养基最优配方为:葡萄糖20g/L,玉米浆20g/L,酵母膏20g/L,K2HPO4 2.5g/L.种子液最佳培养时间为24h,发酵培养基初始pH 5.8,接种量为8%,装液量200mL/1000mL,摇床转速为180 r/min,28℃,底物投料时间24h,发酵结束时间72 h.结论:将该工艺在7L发酵罐中放大,菌体密度达到25.36g/L,11α羟基坎利酮的转化率为86.1%.
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| 关 键 词： | 赭曲霉 高密度培养 11α羟基化坎利酮 转化率 |
Effect of High Cell -Density Cultivation of Aspergillus ochraceus on Bioconversion of Canrenone |
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| LIU Xiao,LIU Yi-han,BIE Song-tao,XU Xing,XIE Jin-xia,LI Jian-xun,HAN Kai-lin,LU Fu-ping.Effect of High Cell -Density Cultivation of Aspergillus ochraceus on Bioconversion of Canrenone[J].Biotechnology,2011,21(5). |
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| Authors: | LIU Xiao LIU Yi-han BIE Song-tao XU Xing XIE Jin-xia LI Jian-xun HAN Kai-lin LU Fu-ping |
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| Institution: | LIU Xiao,LIU Yi-han,BIE Song-tao,XU Xing,XIE Jin-xia,LI Jian-xun,HAN Kai-lin,LU Fu-ping*(Key Laboratory of Industrial Fermentation Microbiology,National Engineering Laboratory for Industrial Enzymes Tianjin Key Laboratory of Industrial Microbiology,College of Biotechnology,Tianjin University of Science & Technology,Tianjin 300457) |
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| Abstract: | Objective:The optimum medium and culture conditions of Aspergillus ochraceus in high-density culture were studied to achieve the high bioconversion rate of canrenone.Method: We chose a kind of cheap and facile medium and optimized its ingredients and other fermentation conditions.Then we got optimum medium ingredients and culture conditions.Result: The optimum medium is: glucose 20g/L,corn steep liquor 20g/L,yeast extract 20g/L,K2HPO4 2.5g/L,other suitable fermentation conditions include: cultivation time o... |
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