| 不同碳氮源对胶质芽孢杆菌GSY -1生物脱硅的影响 |
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| 引用本文： | 惠明,侯银臣,田青,尚小利.不同碳氮源对胶质芽孢杆菌GSY -1生物脱硅的影响[J].生物技术,2011,21(5). |
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| 作者姓名： | 惠明 侯银臣 田青 尚小利 |
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| 作者单位： | 1. 河南工业大学生物工程学院,河南郑州450001,China
2. 河南工业大学生物工程学院,河南郑州,450001 |
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| 基金项目： | 河南省高校青年骨干教师资助项目，河南工业大学校基金招标项目 |
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| 摘 要： | 目的:探讨不同碳氮源对胶质芽孢杆菌GSY -1的生物脱硅效果的影响.方法:采用单因素试验并结合方差分析确定影响GSY -1生物脱硅的碳氮源种类及浓度,通过回归实验及响应面分析进一步优化培养条件,然后利用10L发酵罐进行放大实验验证.结果:研究发现,乳糖和尿素对GSY -1生物脱硅的影响均显著,其中尿素10g/L及乳糖13g/L时,培养液中铝硅比(A/S)分别可由2.84提高到5.45和5.42,通过响应面优化实验,确定尿素10.35g/L、乳糖12.60g/L时,菌株GSY -1的脱硅效果最佳,铝硅比由2.84提高到5.67,增幅达99.65%.经10 L发酵罐放大实验,测得浸矿后的铝硅比为5.07,较浸矿前提高78.52%.结论:乳糖和尿素对胶质芽孢杆菌GSY -1脱硅效果影响显著,响应面法可有效用于GSY -1菌株脱硅条件的优化.
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| 关 键 词： | 胶质芽孢杆菌 铝土矿 脱硅 碳氮源 培养条件 |
Effects of Different Carbon and Nitrogen Sources on Biological Desilication by Bacillus mucilaginosus |
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| HUI Ming,HOU Yin-chen,TIAN Qing,SHANG Xiao-li.Effects of Different Carbon and Nitrogen Sources on Biological Desilication by Bacillus mucilaginosus[J].Biotechnology,2011,21(5). |
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| Authors: | HUI Ming HOU Yin-chen TIAN Qing SHANG Xiao-li |
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| Institution: | HUI Ming,HOU Yin-chen,TIAN Qing,SHANG Xiao-li(College of Biological Engineering,Henan University of Technology,Zhengzhou 450001,China) |
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| Abstract: | Objective:The effects of different carbon sources and nitrogen sources on Bacillus mucilaginosus GSY-1 desilication were researched.Method: The types and concentration of carbon and nitrogen sources of impacting GSY-1 biodesilication were determined by single factor test and analysis of variance,the culture conditions were further optimized by regression experiments and response surface analysis,and then the 10L fermenter was use to amplify experiments.Result:The study found the effects of that lactose and ... |
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| Keywords: | Bacillus mucilaginosus Bauxite desilication carbon and nitrogen source culture conditions |
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