| 苹果斑点落叶病致病菌的鉴定及生物学特性研究 |
| |
| 引用本文： | 张彩霞,陈莹,李壮,康国栋,张利义,周宗山,丛佩华.苹果斑点落叶病致病菌的鉴定及生物学特性研究[J].生物技术,2011,21(4). |
| |
| 作者姓名： | 张彩霞 陈莹 李壮 康国栋 张利义 周宗山 丛佩华 |
| |
| 作者单位： | 1. 农业部果树种质资源利用重点开放实验室,辽宁兴城125100;中国农业科学院果树研究所,辽宁兴城125100
2. 中国农业科学院果树研究所,辽宁兴城,125100 |
| |
| 基金项目： | 国家自然科学基金项目，中央级公益性科研院所基本科研业务费专项课题，现代苹果产业技术体系 |
| |
| 摘 要： | 目的:明确l株分离于感病苹果株系群体的供试菌株AML0801的种类,探讨其生物学特性,为苹果抗病分子育种研究奠定基础.方法:通过形态特征描述,显微形态、超微结构观察等方法,从形态学上明确AML0801的分类.室内模拟侵染试验,了解AML0801致病性.ITS序列测定分析辅助验证供试菌株AML0801.结果:菌株AML0801的分生孢子、产孢表型等形态特征,符合苹果链格孢(Alternaria mali Roberts,A.mali)种的描述,ITS序列分析表明,AML0801与A.mali标准菌株同源性达99％.通过比较病原菌侵染试验证实AML0801具备一定的致病性.结论:结合供试菌株AML0801形态特征、ITS序列分析结果,以及室内模拟的致病性研究,可将供试菌株AML0801鉴定为苹果链格孢(A.mali).
|
| 关 键 词： | 苹果斑点落叶病 致病菌 生物学特性 鉴定 |
Identification and Biological Characteristics of a Pathogenic Strain Causing Alternaria Blotch of Apples |
| |
| ZHANG Cai-xia,CHEN Ying,LI Zhuang,KANG Guo-dong,ZHANG Li-yi,ZHOU Zong-shan,CONG Pei-hua.Identification and Biological Characteristics of a Pathogenic Strain Causing Alternaria Blotch of Apples[J].Biotechnology,2011,21(4). |
| |
| Authors: | ZHANG Cai-xia CHEN Ying LI Zhuang KANG Guo-dong ZHANG Li-yi ZHOU Zong-shan CONG Pei-hua |
| |
| Institution: | ZHANG Cai-xia1,2,CHEN Ying1,LI Zhuang2,KANG Guo-dong1,ZHANG Li-yi1,ZHOU Zong-shan2,CONG Pei-hua1,2(1.Key Laboratory of Fruit Germplasm Resources Utilization,Ministry of Agriculture,Xingcheng 125100,China,2.Research Institute of Pomology,Chinese Academy of Agricultural Sciences,China) |
| |
| Abstract: | Objective:This study is to define a test strain AML0801 founded from susceptible apple cultivars,and explore its biological characteristics,in order to providing some basis for future study about disease-resistant apple varieties.Method:Through the observations on morphological characters,microscopic images,and SEM analysis,classify the test strain AML0801 from morphology.Investigating the pathogenicity of strain AML0801 by some inoculation experiments.The analysis of ITS sequencing of AML0801 was used to i... |
| |
| Keywords: | Alternaria blotch pathogenic strain biological characteristics identification |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
