| 产有机酸采油菌的筛选及产酸情况的分析 |
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| 引用本文： | 康宏,郭艾琳,马梦彪,曹凤麟,李胜贤,杨光,王靖然.产有机酸采油菌的筛选及产酸情况的分析[J].生物技术,2011,21(4). |
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| 作者姓名： | 康宏 郭艾琳 马梦彪 曹凤麟 李胜贤 杨光 王靖然 |
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| 作者单位： | 1. 黑龙江大学生命科学学院,黑龙江哈尔滨,150080
2. 山东鲁抗生物制造有限公司,山东济南,272000 |
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| 基金项目： | 黑龙江大学校青年基金项目 |
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| 摘 要： | 目的:从大庆油田原油样品中筛选出2株产有机酸量较高的菌株,并对其产物进行分析.方法:根据形态特征、生理生化性质和16S rDNA序列分析对菌株进行鉴定,并运用GC/MS法对发酵液进行分析.结果:经鉴定这两株为枯草芽孢杆菌,菌株T10 -3的发酵液中含有乙酸11.407％,异丁酸9.375％,丁二醇79.217％；菌株DH -2 -l发酵液的中含有异丁酸41.56％,丁二醇46.619％,异戊酸4.138％,异庚酸10.680％.结论:这两株细菌在微生物采油方面均有良好的应用前景.
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| 关 键 词： | 微生物采油 16S rDNA 有机酸 气质联仪 |
Screening of Organic Acid Yielding Strain and Analysis of Result of Producing Organic Acid |
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| KANG Hong,GUO Ai-lin,MA Meng-biao,GAO Feng-lin,LI Sheng-xian,YANG Guang,WANG Jing-ran.Screening of Organic Acid Yielding Strain and Analysis of Result of Producing Organic Acid[J].Biotechnology,2011,21(4). |
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| Authors: | KANG Hong GUO Ai-lin MA Meng-biao GAO Feng-lin LI Sheng-xian YANG Guang WANG Jing-ran |
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| Institution: | KANG Hong1,GUO Ai-lin1,MA Meng-biao2,GAO Feng-lin1,LI Sheng-xian1,YANG Guang1,WANG Jing-ran1(1.College of Life Science in Heilongjiang University,Harbin 150080,2.Shandong Lukang Biological Manufacturing Co.Ltd,Ji'nan 272000,China) |
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| Abstract: | Objective:Two strains with high production of organic acids were selected from Daqing crude oil samples.Method:The GC/MS method was adopted to detect the type of the organic acid and organic alcohol with low molecular weight in T10-3 and DH-2-1 fermentations.Result:The fermentation of T10-3 is composed of acetic acid(11.407%),isobutyric acid(9.375%),butanediol(79.217%);The fermentation of DH-2-1 is composed of isobutyric acid(41.56%),butanediol(46.619%),isoamyl acid(4.138%) and enanthic acid.Conclusion:The ... |
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| Keywords: | microbial enhanced oil recovery 16S rDNA organic acid gas chromatography-mass spectram |
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