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己酸菌DNA中G—C含量的测定
引用本文:孙芳义,邹碧珍.己酸菌DNA中G—C含量的测定[J].生物技术,1992,2(3):41-43.
作者姓名:孙芳义  邹碧珍
作者单位:黑龙江省应用微生物所 哈尔滨 (孙芳义,邹碧珍),黑龙江省应用微生物所 哈尔滨(厉人伟)
摘    要:利用核酸的增色性,采用备有加热装置的紫外分光光度计,测定了己酸菌DNA的热变性温度(Tm),得出己酸菌G-C克分子百分数含量为30.9.测定结果表明G-C含量与传统分类允许的范围一致.

关 键 词:己酸菌  增色性  解链温度

Determination of G-C Content in Clostridium Kluvveri
Sun Fangyi Zou Bizhen Li Renwei.Determination of G-C Content in Clostridium Kluvveri[J].Biotechnology,1992,2(3):41-43.
Authors:Sun Fangyi Zou Bizhen Li Renwei
Institution:Institute of Applied Microbiology Heilongjiang Academy of Sciences Harbin
Abstract:useing the hyperchromicity of nuclic acid to measure the thermal denaturat-ion temperature of the double stranded DNA with ultraviolet spectrophotonie-ter equiped with heating apparatus, the determined G-C content of Clostridium Kluyveri was 30.9 The results obtained was consistent with traditional bacterial taxonomy method.
Keywords:Clostridium Kluyveri  Hyperchromicity  Melting temperature
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