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| 油菜试管苗玻璃化发生机理的研究初探 | |
| 引用本文： | 王艳,曾幼玲,贺宾,李金耀,高燕,张富春. 油菜试管苗玻璃化发生机理的研究初探[J]. 生物技术, 2006, 16(1): 75-77 |
| 作者姓名： | 王艳 曾幼玲 贺宾 李金耀 高燕 张富春 |
| 作者单位： | 1. 新疆生物资源基因工程重点实验室,新疆大学生命科学与技术学院,新疆,乌鲁木齐,830046;新疆农业科学院经济作物研究所,新疆,乌鲁木齐,830000 2. 新疆生物资源基因工程重点实验室,新疆大学生命科学与技术学院,新疆,乌鲁木齐,830046 3. 新疆农业科学院经济作物研究所,新疆,乌鲁木齐,830000 |
| 基金项目： | 国家“863”项目(No.2004AA227110-2)，国家科技攻关项目(No.2001BA901A32)资助 |
| 摘 要： | 研究了影响甘蓝型油菜离体下胚轴再生过程中试管苗玻璃化产生的几个因子,试验发现:调整激素6-BA与NAA的浓度、增加蔗糖的浓度提高渗透势对玻璃化无影响;降低培养基中NH4 浓度不仅没有有效地防止玻璃苗的发生反而降低了芽的诱导率;采用牛皮纸代替聚乙烯塑料膜作为封口材料的措施受到了地区的限制,没有得到有效的统计数据;选择1%的琼脂浓度可使玻璃化率显著降低55%,但诱芽率在一定程度上降低了6.9%。 |
| 关 键 词： | 油菜下胚轴 玻璃化 影响因素 诱芽 |
| 文章编号： | 1004-311X（2006）01-0075-03 |
| 收稿时间： | 2005-06-20 |
| 修稿时间： | 2005-10-25 |
Study on the Vitrification Mechanism of Rape Hypocotyl in vitro |
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| WANG Yan,ZENG You-ling,HE Bin,LI Jin-yao,GAO Yan,ZHANG Fu-chun. Study on the Vitrification Mechanism of Rape Hypocotyl in vitro[J]. Biotechnology, 2006, 16(1): 75-77 | |
| Authors: | WANG Yan ZENG You-ling HE Bin LI Jin-yao GAO Yan ZHANG Fu-chun |
| Affiliation: | 1. Key Laboratory of Biological Resources and Genetic Engineering of Xinjiang, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China;2. Institute of Industrial Crops, Xinjiang Academy of Agricultural Sciences, Umrnqi 830000, China |
| Abstract: | Some factors affecting vitrification of rape in vitro were studied.The results showed there were no effects by adjusting concentration of 6-BA and NAA and improving sucrose concentration to increase osmosis.The reduction in NH_4NO_3 dose couldn't solve the vitrification effectively and lowered the rate of inducing shoots.Since the method that polyethylene film as the sealed material of conical flask was substituted for kraft paper was restricted by region climate, valid date couldn't be attained.1% concentration agar could reduce vitrification by 55%,however,at the same time reducing the inducing shoot by 6.9%. |
| Keywords: | hypocotyl vitrification effective factors inducing shoots |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |