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| 携胰岛素调控基因系统的载体构建及鉴定 | |
| 引用本文： | 吴志香,袁凤山,王大会,邵敬伟.携胰岛素调控基因系统的载体构建及鉴定[J].生物技术,2004,14(5):21-23. |
| 作者姓名： | 吴志香 袁凤山 王大会 邵敬伟 |
| 作者单位： | 1. 辽宁中医学院西医诊断教研室,辽宁,沈阳,110032 2. 中国医科大学第一临床学院内分泌,辽宁,沈阳,110001 3. 福州大学化工学院,福建,福州,350002 |
| 基金项目： | 辽宁省自然科学基金资助项目 (962 2 93) |
| 摘 要： | 目的：构建受胰岛素、葡萄糖双向调节的胰岛索分泌调控基因的载体。方法：1．用酚氯仿抽提法提取SD大鼠肝脏基因组DNA，用P1、P2为引物。PCR扩增胰岛素生长因子结合蛋白-1启动子(IGFBP-1)；2.选用pUC118作为克隆载体，用分子克隆技术亚克隆三倍体葡萄糖反应元件(GLRE)3；3，运用基因重组技术将ICFBP-1启动子定向插入(GLRE)3的下游。结果：1.PCR扩增出420bp的目的DNA片断，与文献IGFBP-1 DNA大小一致；2．重组体序列分析显示，克隆的基因序列和文献报道的(GLRE)3及IGFBP-1基因序列一致。结论：成功构建携胰岛素调控基因系统的载体pUC118(GLRE)3-IGFBP-1。为1型糖尿基因治疗的进一步实验研究奠定了基础。 |
| 关 键 词： | 1型糖尿病 胰岛素生长因子结合蛋白1 葡萄糖反应元件 基因治疗 |
| 文章编号： | 1004-311X(2004)05-0021-03 |
| 修稿时间： | 2004年5月28日 |
Construction and Identification of Recombinant Vector Carring Conditioner for Human Proinsulin Gene |
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| WU Zhi-xiang,YUAN Feng-shan,WANG Da-hui,SHAO Jing-wei.Construction and Identification of Recombinant Vector Carring Conditioner for Human Proinsulin Gene[J].Biotechnology,2004,14(5):21-23. | |
| Authors: | WU Zhi-xiang YUAN Feng-shan WANG Da-hui SHAO Jing-wei |
| Institution: | WU Zhi-xiang~1,YUAN Feng-shan~2,WANG Da-hui~2,SHAO Jing-wei~3 |
| Abstract: | Objective:To construct an insulin gene regulated system,which can been stimulated by glucose and inhibited by insulin,in order to lay groundwork for constructing regulated insulin expression vector.Methods:The inhibitory insulin response element of the insulin-like growth factor binding protein-1 (IGFBP-1) basal promoter was amplified by PCR,Choosing pUC118 as the clone vector,we constructed three copies of GLRE by sub-clone technology,and then inserted (GLRE)3 directly upstream of IGFBP-1 by recombinant technology.Results:The DNA sequences of the recombinant gene(pUC118(GLRE)3-IGFBP-1) was the same as reported previously.Conclusion:Construction of recombinant vector caring conditioner for human proinsulin gene are obtained. |
| Keywords: | Type 1 Diabetes Mellitus IGFBP-1 GLRE Gene therapy |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |