| 细胞固定化载体比表面的测定 |
| |
| 引用本文： | 龙中儿,黄运红,蔡昭铃,丛威,欧阳藩.细胞固定化载体比表面的测定[J].生物技术,2003,13(4):21-23. |
| |
| 作者姓名： | 龙中儿 黄运红 蔡昭铃 丛威 欧阳藩 |
| |
| 作者单位： | 1. 中国科学院过程工程研究所,生化工程国家重点实验室,北京,100080
2. 江西师范大学生命科学学院,江西,南昌,330027 |
| |
| 基金项目： | 国家自然科学基金重点资助项目("低品位复杂矿生物冶金的基础研究",59834150) |
| |
| 摘 要： | 为了衡量细胞固定化载体的性能。基于单分子层吸附理论,利用溶液中亚甲蓝染料在固形物表面的吸附倾向;建立了用于测定细胞固定化载体比表面的“动态染料吸附法”,方法学考察时以PVA-海藻酸钠的混合载体为例,结果表明四批次测量同一载体比表面的结果变异系数为5.5%,测量的载体比表面能精确反映载体内PVA,或是海藻酸钠浓度的变化,说明方法重复性好,灵敏度高,同时讨论了文献中“染料吸附法”测定比表面的不足。
|
| 关 键 词： | 细胞固定化载体 比表面 亚甲蓝 单分子层吸附 |
| 文章编号： | 1004-311X(2003)04-0021-03 |
| 修稿时间： | 2003年2月19日 |
A Modified Method to Measure the Specific Surface of Carrier for Cell Immobilization |
| |
| LONG Zhong-er ,HUANG Yun-hong ,CAI Zhao-ling ,CONG Wei ,OUYANG Fan.A Modified Method to Measure the Specific Surface of Carrier for Cell Immobilization[J].Biotechnology,2003,13(4):21-23. |
| |
| Authors: | LONG Zhong-er HUANG Yun-hong CAI Zhao-ling CONG Wei OUYANG Fan |
| |
| Institution: | LONG Zhong-er 1,HUANG Yun-hong 2,CAI Zhao-ling 1,CONG Wei 1,OUYANG Fan 1 |
| |
| Abstract: | In order to examine the property of carriers for cell immobilization,a modified method of measuring its specific surface was developed based on the theory of single-layer molecular absorption of methylene blue on solid surface,the absorbed methylene blue was used to calculate the specific surface at the state of absorbing equilibrium.The reliability of the new method was proved by the experiments on measuring the specific surface of mixed carriers of Poly(vinyl alcohol) and sodium alginate,the coefficient of variation, based on the results for the same carriers,was only 5.5 percent,and the measured specific surface varied with the concentration of contained Poly(vinyl alcohol) or sodium alginate.Also,the defects about this field in literature were discussed as well. |
| |
| Keywords: | specific surface carrier for cell immobilization methylene blue |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
