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大豆中L34基因的克隆与表达分析
引用本文:李淑艳,王建中.大豆中L34基因的克隆与表达分析[J].生物技术,2009,19(4):3-6.
作者姓名:李淑艳  王建中
作者单位:1. 北京林业大学生物学院,北京,100083;孝感学院生命科学技术学院,湖北,孝感,432000
2. 北京林业大学生物学院,北京,100083
摘    要:目的:采用分子手段试图分离与大豆种子低温吸胀相关的基因.方法:利用cDNA-AFLP(cDNA-amplified fragment length polymorphism)和RACE技术从大豆种子胚轴中克隆到一个编码132 kD的全长核糖体蛋白基因,命名为SOL34.结果:序列分析表明,SOL34和苜蓿(gi | 113205273 |)、茄科植物(gi | 48057670 |)及拟南芥(gi | 2500376 |)中L34蛋白基因的同源性分别为95%、95%和90%.半定量RT-PCR结果表明:大豆种子在4℃下吸胀24h内,胚轴中的SOL34被诱导表达,其中当种子低温吸胀6h,SOL34表达量升高非常明显,18h的表达量最大,24h表达量和18h相似;SOL34在大豆不同部位的表达不同,4叶期的大豆幼苗经过低温处理后,根尖中SOL34的表达比非处理材料增强约5倍,同时比胚轴中高约2倍;但是在叶片中,SOL34表达量并没有受到温度的影响,表达量也很弱,说明SOL34在叶片中是组成型表达.结论:结果分析表明,SOL34可能和大豆根的代谢有关.

关 键 词:大豆  L34基因  克隆  表达

Cloning and Expressing Profile Analysis of L34 Gene Isolated from Soybean (Glycine max L. Meer.)
LI Shu-yan,WANG Jian-zhong.Cloning and Expressing Profile Analysis of L34 Gene Isolated from Soybean (Glycine max L. Meer.)[J].Biotechnology,2009,19(4):3-6.
Authors:LI Shu-yan  WANG Jian-zhong
Institution:LI Shu-yan1,2,WANG Jian-zhong1*(1.College of Biological Sciences and Biotechnology,Beijing Forestry University,Beijing 100083,China,2.College of Life Science and Technology,Xiaogan University,Xiaogan 432000,China)
Abstract:Objective: In this experiment,molecular approach was used to isolate gene relevant to imbibition under low temperature in soybean seeds.Method: A ribosomal protein(r-protein) L34-like gene,designed as SOL34 was isolated from soybean by a cDNA-amplified fragment length polymorphism(cDNA-AFLP) technique.Result: The full length cDNA sequence of SOL34 was 360bp encoding a protein with a calculated molecular mass of 132 kD.Compared with proteins in Genbank using the blastp tool,the SOL34 had 95%,95% and 90% sequ...
Keywords:soybean  L34 gene  clone  express  
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