| 用一种改进的电泳方法测定抗冻蛋白的分子量 |
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| 引用本文： | 邹毅Tao 祝沛平.用一种改进的电泳方法测定抗冻蛋白的分子量[J].生物技术,1999,9(4):36-39. |
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| 作者姓名： | 邹毅Tao 祝沛平 |
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| 作者单位： | 浙江大学西溪校区生命科学院 |
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| 摘 要： | 针对抗冻蛋白分子量较小的特点，该文采用了一种改进的Ｔｒｉｃｉｎｅ－ＳＤＳ－ＰＡＧＥ法测定其分子量。采用三层胶系统并在电极缓冲液中以Ｔｒｉｃｉｎｅ代替Ｇｌｙｃｉｎｅ。
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| 关 键 词： | 抗冻蛋白，Tricine－SDS－PAGE法 |
TESTING OF MOLECULAR WEIGHT OF ANTIFREEZEPROTEIN BY AN IMPROVED
ELECTROPHORESIS TECHNIQUE |
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| Zou Yitao,Zhu Peiping,Zhao Xiaoli.TESTING OF MOLECULAR WEIGHT OF ANTIFREEZEPROTEIN BY AN IMPROVED
ELECTROPHORESIS TECHNIQUE[J].Biotechnology,1999,9(4):36-39. |
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| Authors: | Zou Yitao Zhu Peiping Zhao Xiaoli |
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| Abstract: | An improved tricine sodium dodecyl
sulfate polyacrylamide gel electrophoresis (Tricine-SDS-PAGE) technique was used to test the
molecular weight of AFPs. A three-layer gel system was used and the glycine was insteaded by
tricine in the buffer. |
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| Keywords: | antifreeze protein tricine-SDS-PAGE |
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