家蚕核型多角体病毒对小鼠的易感性及其蛋白组份的检测

家蚕核型多角体病毒(Bm-NPV)是一类环状DNA病毒。该病毒作为载体,应用在杆状病毒-昆虫真核生物表达系统。为确定家蚕基因工程表达系统产品对人的安全性,观察了该病毒对哺乳动物细胞株及对小鼠的易感性,建立了检测Bm-NPV蛋白组份的dot-ELISA方法,可用于生物表达器生产的产品中残存多角体病毒组份的检测。结果表明,芽生型多角体病毒(BV)在杂交瘤细胞和HL60细胞中不增殖。不同剂量包埋型多角体病毒(OV)灌胃感染小鼠,小鼠肝、肾组织切片的电镜观察及免疫组化染色未见到病毒颗粒。

STUDIES ON SUSCEPTIBILITY OF BOMBYX MORINUCLEAR POLYHEDROSIS VIRUS TO MICE AND LEFTOVER OF PROTEIDIC PORTIONS OF THE VIRUS

Bombyx Morinuclear Polyhedrosis Virus(Bm-NPV)is a kind of circular DNA virus.It is applied as a carrier in expression system of granulosis virus insect eucaryotic biology.In order to decide the safety of the virus to human,The susceptibility of Budded Virus(B.V)to cell strains and mice,occuluded virus(O.V)in the sheets of liver and renes was observed with microscope and electronic microscope.Polyclonal antibody to the Bm-NPV proteidic portions was established. The results indicate that the B.V is not proliferative in the hydridomal cells and HL60 cells and that O.V can not infect the mice by oral administration.