| 抗生素对谷子外植体的影响 |
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| 引用本文： | 王节之,孙毅,郝晓芬,郑向阳,王潞英.抗生素对谷子外植体的影响[J].生物技术,2000,10(2):6-9. |
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| 作者姓名： | 王节之 孙毅 郝晓芬 郑向阳 王潞英 |
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| 作者单位： | 1. 山西省农科院谷子研究所,山西,长治,046011
2. 山西农科院农业生物技术中心,山西,太原,030001 |
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| 摘 要： | 通过抗生素潮耆素（Ｈｍ）和卡拉霉素（Ｋｍ）进行谷子外植体抗体性浓度的筛选，结果说明，不同外植体抗性浓度不同，谷子愈伤分化再生苗阶段，潮霉素与卡拉霉素的选浓度范围为５ｍｇ／Ｌ－１０ｍｇ／Ｌ和１５ｍｇ／ｌ－２０ｍｇ／Ｌ，米粒长苗时其浓度为１５ｍｇ／Ｌ、３０ｍｇ／Ｌ。
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| 关 键 词： | 谷子 潮霉素 卡拉霉素 抗性浓度 转基因育种 |
| 修稿时间： | 1999－09－25 |
The effection of antibiotic substances to millet explant |
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| WANG Jie-zhi,SUN Yi,HAO Xiao-fen,ZHEN Xiao-yong,WANG Lu-ying.The effection of antibiotic substances to millet explant[J].Biotechnology,2000,10(2):6-9. |
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| Authors: | WANG Jie-zhi SUN Yi HAO Xiao-fen ZHEN Xiao-yong WANG Lu-ying |
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| Abstract: | Through screening concentration of Hygrimycin and Kamamycin on millet explant, the result showed that resistance concentration is different from different millet explant. In the stage from the callus to differentiation plantlet. Hygrimycin is 5-10 mg/L, Kamamycin is 15-20mg/L. When hasked millet grows in MS mediem, Hygrimycin is 15mg/L, Kamamycin is 30mg/L. |
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| Keywords: | hasked millet differentiation plantlet from callus hygrimycin kamamycin resistance concentration |
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