| 植物材料表面消毒方法的改进 |
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| 引用本文： | 肖显华,王顺珍,林荣双,郑秋生.植物材料表面消毒方法的改进[J].生物技术,1999,9(1):43-45. |
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| 作者姓名： | 肖显华 王顺珍 林荣双 郑秋生 |
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| 作者单位： | 烟台大学生化系,烟台,264005 |
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| 摘 要： | 苹果枝条用０．１％ＨｇＣＩ2磁力搅拌消毒１５-２０ｍｉｎ，无菌水磁力搅拌清洗５次，每次５ｍｉｎ。污染率和杀死率在１０％以下，成功率９０％左右，且无褐化。
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| 关 键 词： | 植物材料 表面消毒 |
| 修稿时间： | 1998年7月10日 |
IMPROVEMENT ON SURFACE STERILIZATION OF PLANT MATERIALS |
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| Xiao Xianhua, Wang Shunzhen ,Lin Rongshuang ,Zheng Qiusheng.IMPROVEMENT ON SURFACE STERILIZATION OF PLANT MATERIALS[J].Biotechnology,1999,9(1):43-45. |
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| Authors: | Xiao Xianhua Wang Shunzhen Lin Rongshuang Zheng Qiusheng |
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| Institution: | Biochemistry Department;;Yantai University;;Yantai;;264005 |
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| Abstract: | Apple shoots were segmented and submerged in 0.1 % HgCl2 solution. sterilized throughmagnetic stir for 15 - 20 minutes.Then they were cleansed in the same way in sterilizedwater 5 tAnes. every time 5 minutes. The materials were inoculated on MS media andcultured for 10 days. The results showed that under 10% of the cultures were contaminatedand killed respectively while about 90% of the cultures remained alive without contaminationor browning. |
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| Keywords: | plant materials surface sterilization |
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