| 提高水稻组培诱导频率方法及其对耐冷变异体筛选的研究 |
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| 引用本文： | 陈力,吕晓波,刘丽艳,陈香兰,王春燕.提高水稻组培诱导频率方法及其对耐冷变异体筛选的研究[J].生物技术,1991(4). |
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| 作者姓名： | 陈力 吕晓波 刘丽艳 陈香兰 王春燕 |
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| 作者单位： | 黑龙江省农科院生物技术研究中心 哈尔滨
(陈力，吕晓波，刘丽艳，陈香兰)，黑龙江省农科院生物技术研究中心 哈尔滨(王春燕) |
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| 摘 要： | 本试验得到外植体种胚的适宜接种量为6粒左右,培养基量为15—20毫升。由于不同品种具有的基因型各异,因而接种的外植体成熟胚和幼穗离体培养的愈伤组织诱导与分化率也有所不同。在Ms培养基中NAA的适宜浓度为2mg/L,附加一定量的酵母膏有提高分化率的趋势。 从560份耐冷变异体株系中,经生理生化测定和田间鉴定,初步认为耐冷变异体有三份:647—4;160—3—1;和160—4。
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| 关 键 词： | 诱导率 分化率 突变体 |
STUDYING ON THE METHODS OF INCREASING INDUCTION RATE OF RICN TISSUE CULTURE AND SCREENING RICE VARIANTS FOR COLD TOLERANCE |
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| Chen Li Lu XiaoBo Liu YiYau Chen Xianglan Wang Chunyan.STUDYING ON THE METHODS OF INCREASING INDUCTION RATE OF RICN TISSUE CULTURE AND SCREENING RICE VARIANTS FOR COLD TOLERANCE[J].Biotechnology,1991(4). |
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| Authors: | Chen Li Lu XiaoBo Liu YiYau Chen Xianglan Wang Chunyan |
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| Abstract: | On the basis of different varieties with different induction rate and regeneration frquency in tissue culture.The several methods that increase induction rate have been carried out in this experiment. It indicates that appropriate number of inoculation of explant embryo is about 6 seeds when the volume of culture medium is 15-20 ml It also reports that regeneration frequency has been increased by properconcentration of NAA(2mg/L) and additional some yeast extract in MS culture medium |
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| Keywords: | Induction rate Regeneration frequency Variant |
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