| 产1,3-丙二醇菌株丁酸梭菌的诱变育种 |
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| 引用本文： | 李凤梅,刘长江,郑艳,王希尧.产1,3-丙二醇菌株丁酸梭菌的诱变育种[J].生物技术,2005,15(1):34-35. |
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| 作者姓名： | 李凤梅 刘长江 郑艳 王希尧 |
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| 作者单位： | 1. 沈阳农业大学食品学院,辽宁,沈阳,110161
2. 辽宁科技学院生化系,辽宁,本溪,117022 |
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| 摘 要： | 甘油由丁酸梭菌转化成1，3-丙二醇的研究是厌氧条件下进行。为了获得1，3-丙二醇的高产突变株，以丁酸梭菌为出发菌株进行诱变处理。经过硫酸二乙酯（DES）化学诱变得到2株高产正突变株C.but2031和C.but2046，再经过紫外线和亚硝基胍（NTG）复合诱变得到突变株C.but3037。经过初筛、复筛和传代实验，表明其是稳定的突变株。C.but3037的1，3-丙二醇产量由出发菌株的2.2g/L提高到15.7g/L，提高了6.13倍，
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| 关 键 词： | 梭菌 高产 发菌 菌株 突变株 诱变育种 株高 1，3-丙二醇 丁酸 复合诱变 |
| 文章编号： | 1004-311X(2005)01-0034-02 |
| 修稿时间： | 2004年11月21 |
Breeding of Strain Clostridium butyricum Producing 1,3-Propanediol |
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| LI Feng-mei,LIU Chang-jiang,ZHENG Yah,WANG Xi-yao.Breeding of Strain Clostridium butyricum Producing 1,3-Propanediol[J].Biotechnology,2005,15(1):34-35. |
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| Authors: | LI Feng-mei LIU Chang-jiang ZHENG Yah WANG Xi-yao |
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| Institution: | LI Feng-mei1,LIU Chang-jiang1,ZHENG Yan1,WANG Xi-yao2 |
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| Abstract: | The conversion of glycerol to 1,3-propanediol (1,3-PD) by Clostridium butyricum was studied in anaerobic culture.Clostridium butyricum,as original strain,was treated twice by diethyl sulfate and two mutants,C.but 2031 and C.but 2046,were obtained.Then they were treated by multiple mutagenesis UV and N-methyl-N'-nitro-N-nitrosogunidine(NTG).A higher-yield strain C.but 3037 was bred through the primary screening and examination test of 1,3-PD production as the secondary screening.It was showed that the genetic property of this new mutant was stable by pass-generation tests and the yield of 1,3-PD was higher than 15.7g/L,which had been increased about 631% than that of the initial strain. |
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| Keywords: | Clostridium butyricum glycerol fermentation 1 3-propanediol mutation breeding |
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