| 蚯蚓纤溶酶的糖成分分析 |
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| 引用本文： | 张伟,刘秀丽.蚯蚓纤溶酶的糖成分分析[J].生物技术,1997,7(4):19-22. |
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| 作者姓名： | 张伟 刘秀丽 |
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| 作者单位： | 河北大学生物技术研究中心!保定，071002，济南山东大学微生物系，河北大学生物技术研究中心!保定，071002，中科院武汉病毒所，河北大学生物技术研究中心!保定，071002 |
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| 摘 要： | 以大豆胰蛋白酶抑制剂为配基的亲和层析制备的蚯蚓纤溶酶是一组糖蛋白。通过苯酚一硫酸法测中性糖，3，5一二硝基水扬酸法测还原糖，硅胶G薄层层析法鉴定糖的种类，结果为：蚯蚓纤溶酶主要含有中性己糖，含量为15％左右，TFMS的去糖处理会使蚯蚓纤溶酶酶活丢失．
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| 关 键 词： | 蚯蚓纤溶酶 中性已糖 糖蛋白 |
Composition Analysis of Sugar of the Earthworm Fibrinolytic Enzyme |
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| Zhang Wei, Liu Xiuli, Zhao Xiaoyu.Composition Analysis of Sugar of the Earthworm Fibrinolytic Enzyme[J].Biotechnology,1997,7(4):19-22. |
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| Authors: | Zhang Wei Liu Xiuli Zhao Xiaoyu |
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| Institution: | Biotechology reseach enter of Hebei University.Baoding 071002 |
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| Abstract: | The earthworm fibrinolytic enzyme was prapared by affinity chromatography using soy been trypsin inhibitor as a ligand. It was a set of glycoproteins. The protocols used were phenol sulfuric acid method. 3, 5 -dinitrosalicylic acid method and thin-layer chromatography of silic gel G.1 was shown that the suger the enzyme contained was about 15% and mainly neutrol hexose.The enzyme was lost its activty after sugar being deleted with TFMS. |
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| Keywords: | earthworm fibrinolytic enzyme sugar glycoproteins |
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