| 桔青霉中紫杉醇诱导子对红豆杉细胞代谢的影响 |
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| 引用本文： | 李家儒,田廷亮.桔青霉中紫杉醇诱导子对红豆杉细胞代谢的影响[J].生物技术,1997,7(4):23-26,22. |
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| 作者姓名： | 李家儒 田廷亮 |
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| 作者单位： | 华中理工大学生物工程系!武汉，华中理工大学生物工程系!武汉，华中理工大学生物工程系!武汉，河南医科大学，华中理工大学生物工程系!武汉，华中师范大学生命科学学院!武汉 |
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| 摘 要： | 在红豆杉细胞培养第２０ｄ时，加入桔青霉紫杉醇诱导子处理５ｄ及１０ｄ后细胞中可溶性蛋白质含量增加，苯丙氨酸解氨酶活性增强，培养基ｐＨ值降低，细胞可溶性蛋白质，过氧化物酶与酯酶同工酶电泳扫描图谱中出现新的谱带为茂本原有个别谱带强度发生变化。
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| 关 键 词： | 红豆杉 桔青霉 诱导子 细胞培养 代谢 紫杉醇 |
Effects of a Taxol Elicitor from Penicillium citrinum on the metabolism of Taxus chinensis Cells |
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| Li Jiaru, Liu Manix, Cao Mengde, Wang Junjian.Effects of a Taxol Elicitor from Penicillium citrinum on the metabolism of Taxus chinensis Cells[J].Biotechnology,1997,7(4):23-26,22. |
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| Authors: | Li Jiaru Liu Manix Cao Mengde Wang Junjian |
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| Abstract: | A taxol elictiro from Penicillium citrinum could affect the metabolism of Taxus chinensis cells. 5days and 10days after the elicitor was added on the 20th day of the cell suspension culture, the intracellular soluble protein contents and phenylalanine amonia-lyase activityes of the cultured cell incrased, the medium pH values decreased, some extra bands appeared in the scanning curves of the intracellular soluble protein, isoperoxidase and isoesterase patterns and thechanges of the intensities of few bands were observed. |
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| Keywords: | Penicillium citrinum Ebilictor Ebicitor Taxus chinensis Cell culture Metabolic cchanges |
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