香蕉枯萎菌基因组DNA提取方法的研究

以香蕉枯萎菌菌株为试验材料，在SDS～CTAB法和高盐沉淀法等基础上加以改进，对两种提纯香蕉枯萎菌基因组DNA的方法进行了比较研究。结果表明：高盐沉淀法是适合于香蕉枯萎菌基因组DNA提取的方法。该方法提取的DNA OD260／OD280的比值为1．841，DNA产量为0．81mgDNA／g菌丝体。基因组DNA经琼脂糖凝胶电泳得到一条带型较宽且清晰的DNA谱带，基本无DNA碎带；将提取的DNA直接用于PCR扩增，得到带多而且清晰、整齐、基本无拖尾的RAPD图谱。

Study of DNA Extraction Methods in Fusarium oxysporum f.sp.cubense

In the study,SDS-CTAB and High-concentration-salt precipitation methods were used to extract gDNA of Fusarium oxysporum f.sp.cubense.The results of the experiments showed that high-concentration-salt precipitation method was fit for extracting gDNA of Fusarium oxysporum f.sp.cubense.The ratio of their OD_(260)/OD_(280) value of the DNA extracted was 1.841 and the yield per gram mycelium was 0.81mg.The DNA extracted had a clear fragment through agarose gel electrophoresis and do not take RNA without adding RNase and can be directly used in RAPD-PCR amplification without any further purification.