| 被孢霉W_（A－90）发酵生产γ－亚麻酸的研究 |
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| 引用本文： | 张道海,王未名,陆文华,李楠.被孢霉W_（A－90）发酵生产γ－亚麻酸的研究[J].生物技术,1995(1). |
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| 作者姓名： | 张道海 王未名 陆文华 李楠 |
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| 作者单位： | 山东省农科院原子能应用研究所，山东省农科院土壤肥料研究所 |
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| 摘 要： | 本文研究了由被抱霉变株ＭＡ－９０生产了γ－亚麻酸的发酵条件．确定了最适碳源、复源及Ｃ／Ｎ．初步建立了生产γ－亚麻酸的工艺条件。葡萄糖、蔗糖及天冬酰胺和尿素为最适碳、氮源。在Ｃ／Ｎ为２０／１，葡萄糖浓度为８０ｇ／Ｌ的条件下．油脂产量和油脂中γ－亚麻酸的含量分别为８．２ｇ／Ｌ和１４．１３％，γ－亚麻酸产量及生物量分别为１．１５７ｇ／Ｌ和３１．２ｇ／Ｌ。后期适当降低培养温度和良好的通气条件均有利于γ－亚麻酸的积累。
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| 关 键 词： | 亚麻酸 被孢霉 发酵 |
Conditions for Gamma--Lindenic Acid Production by Mortierella sp. M_(A-90) |
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| Zhang Daohai, Wang Weiming, Lu Wenhua.Conditions for Gamma--Lindenic Acid Production by Mortierella sp. M_(A-90)[J].Biotechnology,1995(1). |
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| Authors: | Zhang Daohai Wang Weiming Lu Wenhua |
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| Abstract: | The conditions for Gammau-linolenic acid production by mutant strain MA-90 of Mortierella sp.were studied. The optimum carbon source, nitrogen soutee, C/N ratio And the proeds for GLA accumulation were also determined. The yield Of total lipid and the content of GLA in the fstty acids of total lopids were 8. 20g/L and 14. 13%, respectively. The. maalmum GLA productivity and biomass of 1. 157g/L and 31. 2 g/L were observed for a 80 g/L glucose medium. Decreasing the late--cultivation temperature and good aeration stimulate GLA accumulation in cell. |
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| Keywords: | Gamma-linolenic Acid(GLA) Mortierella sp Wermentation |
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