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| 寡糖对SPF大鼠肠道微生物多样性影响的研究 | |
| 引用本文： | 高启禹,吴襟,徐光翠,段子渊.寡糖对SPF大鼠肠道微生物多样性影响的研究[J].生物技术,2006,16(5):28-30. |
| 作者姓名： | 高启禹 吴襟 徐光翠 段子渊 |
| 作者单位： | 1. 河南新乡医学院生命科学技术系,河南,新乡,453003;中国科学院微生物研究所,北京,100080 2. 中国科学院微生物研究所,北京,100080 3. 河南新乡医学院公共卫生学系,河南,新乡,453003 4. 中国科学院遗传与发育研究所,北京,100101 |
| 基金项目： | 中国科学院知识创新工程重要方向项目资助(No．KSCX2-SW-323)，北京市自然科学基金课题(5982010) |
| 摘 要： | 目的：研究寡糖对SPF大鼠肠道微生物的影响作用。方法：从饲养30d的SPE大鼠盲肠内容物中直接提取总DNA,PCR扩增获得16S rDNA片段,进行DCGE分析。结果：通过DGGE条带数目及亮度的变化,反映出饲喂寡糖能提高和改善肠道内有益菌的数量和组成。结论：饲喂不同水平的寡糖对大鼠肠道微生物菌群影响较大。 |
| 关 键 词： | DGGE 寡糖 肠道 生态因子 |
| 文章编号： | 1004-311X（2006）05-0028-03 |
| 收稿时间： | 2006-04-26 |
| 修稿时间： | 2006-08-22 |
Effect of Oligosaccharide on the Intestinal Microbial Ecosystem of SPF Rats |
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| GAO Qi-yu,WU Jin,XU Guang-cui,DUAN Zi-yuan.Effect of Oligosaccharide on the Intestinal Microbial Ecosystem of SPF Rats[J].Biotechnology,2006,16(5):28-30. | |
| Authors: | GAO Qi-yu WU Jin XU Guang-cui DUAN Zi-yuan |
| Institution: | 1 .Department of Life Science and Technology, Xinxiang Medical College, Xinxiang 453003;2.Institute of Microbiology, Chinese Academy of Science, Beijing 100080; 3. Department of Public Health, Xinxiang Medical College, Xinxiang 453003 ;4. Institute of Genetics, Chinese Academy of Science, Beijing 100101, China |
| Abstract: | Objective:To study the effect of oligosaccharide on the intestinal microorganism of SPF rats.Methods:general DNA was extract from the contents of cecum in the SPF rats which were raised for 30 days,the 16S rDNA fragments were obtained with PCR and were analyzed with De- naturing Gradient Gel Electrophoresis(DGGE).Results:The number and brightness of DGGE band occurred an obvious change,and Reflected feeds the oligosaccharide to be able to enhance and to improve in the intestinal tract the quantity and the composition.Conclusion:Feeding differ- ent levels of oligosaccharide to rats have a larger influence the intestinal microorganism. |
| Keywords: | DGGE oligosaccharide intestinal tract ecological factor |
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