| 奥利亚罗非鱼DMRT1,DMO基因片段的克隆及序列分析 |
| |
| 引用本文： | 唐永凯,王光花,吴婷婷.奥利亚罗非鱼DMRT1,DMO基因片段的克隆及序列分析[J].生物技术,2006,16(2):1-3. |
| |
| 作者姓名： | 唐永凯 王光花 吴婷婷 |
| |
| 作者单位： | 1. 中国水产科学研究院淡水渔业研究中心,江苏,无锡,214081
2. 莱阳农学院水产学院,山东,青岛,266109 |
| |
| 基金项目： | 国家“863”项目(2001AA243061)，国家自然科学基金项目(30371116)，无锡市自然科学基金项目(CK030001) |
| |
| 摘 要： | 根据其他鱼类DMRT基因中的保守序列设计了一对简并引物,利用RT-PCR扩增了雌雄奥利亚罗非鱼的DMRT基因,并对其扩增产物进行了克隆与测序。结果在雌雄奥利亚罗非鱼个体中获得了两个不同的片段,分别命名为DMO,DMRT1基因。序列同源性分析表明,奥利亚罗非鱼DMRT1,DMO cNA系列的同源性为61.78%,氨基酸同源性为86%,说明了DMRT基因存在性别差异。与尼罗罗非鱼、红鳍东方豚、虹鳟、青鱼将等鱼类DM保守区相比,氨基酸序列同源性为86%-100%,这充分显示了DM-RT基因在进化上的高度保守性。
|
| 关 键 词： | 奥利亚罗非鱼 DM保守区 DMRTl基因 DMO基因 |
| 文章编号： | 1004-311X（2006）02-0001-03 |
| 收稿时间： | 2005-12-21 |
| 修稿时间： | 2006-01-12 |
cDNA Fragment Clone and Sequence Analysis of dmrt1, dmo Gene in Tilapia, Oreochromis aurea |
| |
| TANG Yong-kai,WANG Guang-hua,WU Ting-ting.cDNA Fragment Clone and Sequence Analysis of dmrt1, dmo Gene in Tilapia, Oreochromis aurea[J].Biotechnology,2006,16(2):1-3. |
| |
| Authors: | TANG Yong-kai WANG Guang-hua WU Ting-ting |
| |
| Institution: | 1. Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wind 214081, China; 2. Dept. of Hydrobiology and Engineering, LAC, Laiyang 265200, China |
| |
| Abstract: | Using a pair of degenerate primers,We amplified two 158bp cDNA fragments of the dmrt gene in tilapia,Oreochromis aurea from testis and ovary by RT-PCR.The sequences from male and female tilapia were different.We named them dmrt1,and dmo gene respectively.The sequence analysis indicated that the cDNA sequence and the amino acids homogeneity between dmrt1 and dmo gent were 61.78% and 86% respectively,which showed that dmrt gene was different in male and femal tilapia.We compared the alignment of deduced amino acid sequences between DMO cDNA from O.aurea,O niloticus and DMRT1 cDNA from O.niloticus,fugu,rainbow trout,medaka.The score was 86%-100%.These results indicated that DMO and DMRT1 genes were highly conservative in phylogeny. |
| |
| Keywords: | Oreochromis aurea DM-domain dmrt1 dmo |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
