| 应用实时荧光PCR技术定性定量检测改良品质的转基因小麦 |
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| 引用本文： | 栾凤侠,张洪祥,白月.应用实时荧光PCR技术定性定量检测改良品质的转基因小麦[J].生物技术,2007,17(4):48-51. |
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| 作者姓名： | 栾凤侠 张洪祥 白月 |
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| 作者单位： | 黑龙江出入境检验检疫局,黑龙江,哈尔滨,150001 |
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| 基金项目： | 国家质量监督检验检疫总局科研项目 |
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| 摘 要： | 目的:对转入高分子量谷蛋白亚基的小麦中转基因成分进行实时荧光PCR定性定量检测。方法:针对转基因小麦品系中通用的ubiquitin启动子,NOS终止子以及标记基因bar基因进行定性筛选检测,同时用已知为单拷贝的Wx012基因作为小麦物种内源特异参照基因,用单粒B73转基因小麦提取基因组DNA建立内源基因和外源基因的标准曲线,对转基因小麦样品A进行定量检测,同时优化实时荧光PCR条件反应条件。定量检测结果为5.35%。结果:研究的实时荧光PCR技术对转基因小麦中转基因成分能够快速准确地进行定性定量检测。
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| 关 键 词： | 转基因小麦 实时荧光PCR 定性检测 定量检测 |
| 文章编号： | 1004-311X（2007）04-0048-04 |
| 收稿时间： | 2007-01-04 |
| 修稿时间： | 2007-03-28 |
Qualitative, Quantitative Detecting of Genetically Modified Wheat by Real- time PCR |
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| LUAN Feng-xia,ZHANG Hong-xiang,BAI Yue.Qualitative, Quantitative Detecting of Genetically Modified Wheat by Real- time PCR[J].Biotechnology,2007,17(4):48-51. |
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| Authors: | LUAN Feng-xia ZHANG Hong-xiang BAI Yue |
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| Institution: | Heilongjiang Entry- Exit Inspection and Quarantine Bureau,Harbin 150001 ,China |
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| Abstract: | Objective:This study is about qualitative(quantitative) detecting of genetically modified wheat by real-time PCR.Method:Primers and probes were designed to amplify parts of ubiquitin,NOS and bar genes which are universal in wheat.Good amplified curves were obtained.At the same time,the Wx012 gene was used as endogenesis and amplified successfully.Standard curves were made by B73 wheat,the result of qualitative detecting of 5% contend transgenic wheat was 5.35%.Results: This method can qualitative(quantitative) detecting of genetically modified wheat. |
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| Keywords: | GMO Wheat Real-time PCR Qualitative detecting |
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