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| 透明颤菌(沈阳株)血红蛋白基因克隆与序列分析 | |
| 引用本文： | 张志强,吴琼,王洪军,孙艳,冯立,王立强,吴益民.透明颤菌(沈阳株)血红蛋白基因克隆与序列分析[J].生物技术,2005,15(6):1-2. |
| 作者姓名： | 张志强 吴琼 王洪军 孙艳 冯立 王立强 吴益民 |
| 作者单位： | 1. 沈阳军区联勤部疾病预防控制中心,辽宁,沈阳,110034 2. 吉林大学药学院,吉林,长春,130062 3. 沈阳药科大学,辽宁,沈阳,1100 |
| 摘 要： | 目的：以透明颤菌（Vitreoscilla）基因组DNA为模板，扩增透明颤菌血红蛋白基因（vgb）。方法：采用PCR81与摹甲季缉彗术，将PCR产物插入到克隆载体PET28a中，测序应用DNA测序仪。结果：获得了约0．5kbDNA片段，测序结果与已发表的透明颤菌血红蛋白基因核苷酸序列比较，同源性为97％。结论：所扩增的DNA片段确认是透明颤菌血红蛋白基因。 |
| 关 键 词： | 透明颤菌 血红蛋白基因 序列分析 |
| 文章编号： | 1004-311X（2005）06-0001-02 |
| 收稿时间： | 2005-08-19 |
| 修稿时间： | 2005-09-20 |
Cloning and Sequencing of Vitreoscilla (Shenyang strain )Hemoglobin Gene |
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| ZHANG Zhi-qiang,WU Qiong,WANG Hong-jun,SUN Yan,FENG Li,WANG Li-qiang,WU Yi-min.Cloning and Sequencing of Vitreoscilla (Shenyang strain )Hemoglobin Gene[J].Biotechnology,2005,15(6):1-2. | |
| Authors: | ZHANG Zhi-qiang WU Qiong WANG Hong-jun SUN Yan FENG Li WANG Li-qiang WU Yi-min |
| Institution: | 1. Centre of Disease Prevent and Control of Shenyang Command, Shenyang 110034; 2. College of Midecine Jinlin University, Changchun 130062; 3. Shenyang Medical University, Shenyang 110000, China |
| Abstract: | Objective:Vitreoscilla genomic DNA is used template and amplifing vgb.Methods:By Polymerase chain reaction-PCR and Gentic Recombinant technique,Then PCR production ligated with vector PET28a.DNA sequencing by DNA sequencer.Results:It were successfully used to amplify 0.5kb hemoglobin gene,and the homology was 97% compared with the published vge gene framents.Conclusion:This study amplifying DNA was confirmed Vitreoscilla vgb. |
| Keywords: | Vitreoscilla Hemoglobin gene quence analsis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |