河套蜜瓜ACC合成酶cDNA片段的克隆和序列分析

1-氨基环丙烷-1-羧酸（ACC）合成酶是高等植物中乙烯生物合成的关键酶。以成熟河套蜜瓜（CucumismeloL．cvHetau）果实的RNA为模板，经反转录和PCR扩增得到预期大小的DNA片段，插入到pUC19的SmaⅠ位点后转化E.coliJM109，筛选出重组子pHMAS1。序列分析表明获得了长627bp的ACC合成酶cDNA片段。与已报道的ACC合成酶基因相应序列比较有很高的同源性．

Cloning and Sequencing of the cDNA Fragment Encoding ACC Synthase in Hetau melon

l-Aminocyclopropane-l-carboxylic acid (ACC) synthase is the key emzyme in the biosynthasis of ethylene. Totol RNA was isolated from ripe Hetau melon (Cucumis melo L.cv Hetau) fruits. Using oligo(dT)(15) as primer the first strand of cDNA was synthesized and a 0. 63 kb DNA fragment was obtained with PCR technique. This DNA frangment wascloned into Sma I site Of pUC19, then introducted into E. coli JM109. Sequence analysis of the recombinant PHMAS1 demonstrated that a fragment of DNA encoding ACC synthase was obtained. This fragment cousists of 627bp. The DNA sequence and deduced amino acid sequence is highly homologous to that of the ACC synthase cDNA from other plant species.