| 苛求芽孢杆菌基因组DNA提取方法的比较 |
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| 引用本文： | 赵运胜,卜友泉,张宏娟,廖飞.苛求芽孢杆菌基因组DNA提取方法的比较[J].生物技术,2006,16(2):41-42. |
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| 作者姓名： | 赵运胜 卜友泉 张宏娟 廖飞 |
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| 作者单位： | 1. 基础医学院生物化学与分子生物学教研室,重庆医科大学,重庆,400016
2. 检验系临床微生物学教研室,重庆医科大学,重庆,400016 |
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| 基金项目： | 国家自然科学基金项目资助(30200266) |
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| 摘 要： | 目的:比较不同方法提取苛求芽孢杆菌基因组DNA的差异。方法:用经典CTAB提取法、改进CTAB法(溶菌酶处理结合CTAB提取法)、UniQ柱吸附提取法制备苛求芽孢杆菌基因组DNA,比较产物完整性和用于PCR扩增的有效性。结果:三种方法制备基因组DNA纯度接近,但改进CTAB法产率最高,UniQ法产率最低。经典CTAB法和UniQ法提取基因组DNA易降解。三种方法所得基因组DNA用于PCR扩增效率接近。结论:溶菌酶裂解结合CTAB提取更适合制备苛求芽孢杆菌基因组DNA。
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| 关 键 词： | 苛求芽孢杆菌 基因组DNA提取 改进CTAB法 |
| 文章编号： | 1004-311X（2006）02-0041-02 |
| 收稿时间： | 2005-10-25 |
| 修稿时间： | 2006-01-03 |
Comparison of Methods to Prepare Genomic DNA from Baccilus fastidious |
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| ZHAO Yun-sheng,BU You-quan,ZHANG Hong-juan,LIAO Fei.Comparison of Methods to Prepare Genomic DNA from Baccilus fastidious[J].Biotechnology,2006,16(2):41-42. |
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| Authors: | ZHAO Yun-sheng BU You-quan ZHANG Hong-juan LIAO Fei |
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| Abstract: | Objectives:To compare methods for the preparation of genomic DNA from Bacillus fastidious.Methods: Genomic DNA was extracted by classical CTAB method,modified CTAB method with the use of lysozyme and UniQ ion-exchange method,the quality and quantity of genomic DNA were compared.Results: The modified CTAB method showed the highest yield,while the UniQ ion-exchange method showed the lowest yield of genomic DNA.These three methods gave DNA preparation of comparable homogenecity and efficiency as the template for PCR amplification.Conclusion: The modified CTAB method was better for preparing genomic DNA from Bacillus fastidious. |
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| Keywords: | Bacillus fastidious the extraction of genomic DNA modified CTAB method |
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