| 两种富集方法相结合对蓖麻毒素进行SELEX筛选研究 |
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| 引用本文： | 唐吉军,谢剑炜,邵宁生,沈晓书.两种富集方法相结合对蓖麻毒素进行SELEX筛选研究[J].生物技术,2005,15(1):35-37. |
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| 作者姓名： | 唐吉军 谢剑炜 邵宁生 沈晓书 |
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| 作者单位： | 1. 军事医学科学院毒物药物研究所,北京,100850
2. 军事医学科学院基础医学研究所,北京,100850 |
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| 基金项目： | 国家自然科学基金资助项目 (No .2 0 30 50 1 3) |
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| 摘 要： | 为了获得能特异识别具有细胞毒性的蓖麻毒素蛋白寡核苷酸适配子，体外构建了含40个随机序列全长87nt的随机ssDNA文库，采用指数富集配基的系统进化(SELEX)技术方法，结合微孔板和亲和树脂两种分离、富集方法，经过数轮筛选，文库与蓖麻毒素的结合率达到了38．5％。结果表明，以亲和树脂为分离介质进行筛选，富集效果非常明显。
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| 关 键 词： | 指数富集配基的系统进化 蓖麻毒素 随机ssDNA文库 适配子 |
| 文章编号： | 1004-311X(2005)01-0035-03 |
| 修稿时间： | 2004年8月22日 |
SELEX Screening Aptamers for Ricin by Two Enrichment Methods |
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| TANG Ji-jun,XIE Jian-Wei,SHAO Ning-sheng,SHEN Xiao-shu.SELEX Screening Aptamers for Ricin by Two Enrichment Methods[J].Biotechnology,2005,15(1):35-37. |
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| Authors: | TANG Ji-jun XIE Jian-Wei SHAO Ning-sheng SHEN Xiao-shu |
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| Institution: | TANG Ji-jun1,XIE Jian-wei |
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| Abstract: | In order to obtain oligonucleotide aptamers which can specifically recognize the cytotoxin ricin protein,we designed and synthesized an 87 nucleotides ssDNA pool containing 40 random nucleotides flanked by invariant binding regions for PCR primers,which could be used to screen out a specific aptamer for ricin by SELEX (systematic evolution of ligands by exponential enrichment).After several rounds selection,the 38.5% binding ratio of the ssDNA pool bound to ricin protein can be obtained.The results show that the high efficiency of enrichment has been observed by using affinity resin as separation medium. |
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| Keywords: | SELEX Ricin ssDNA pool Aptamer |
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