| 还原型谷胱甘肽高产菌株的初筛及其提取工艺优化 |
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| 引用本文： | 王爽,张惠文,叶淑红,徐明恺.还原型谷胱甘肽高产菌株的初筛及其提取工艺优化[J].生物技术,2011,21(3). |
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| 作者姓名： | 王爽 张惠文 叶淑红 徐明恺 |
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| 作者单位： | 1. 大连工业大学食品学院,辽宁大连,116034
2. 中国科学院沈阳应用生态研究所,辽宁,辽宁沈阳,110016 |
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| 摘 要： | 目的:筛选还原型谷胱甘肽(GSH)高产菌株并优化其提取工艺.方法:从中科院沈阳应用生态研究所菌种保藏室的酵母菌库中筛选GSH高产的酵母菌,改进培养基组分,提高胞内GSH含量,并优化热水抽提和乙醇提取两种方法,提高提取液中GSH含量.结果:筛选获得一株酿酒酵母Y,其胞内GSH含量9.60 mg/g,培养基改良后,胞内GSH含量又提高了34.8%.通过单因素和正交试验确定热水抽提法最优提取条件为:料液比1∶3,pH 2.0,在90℃水浴中,抽提10min,GSH的产量可达14.27mg/g干菌体.结论:添加氨基酸和葡萄糖都有利于酵母菌体的生长和GSH合成.热水抽提法较乙醇提取法相比,提取效果好、无污染、操作简单,为后续的分离纯化工作奠定基础.
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| 关 键 词： | 还原型谷胱甘肽 酿酒酵母 培养基组分 分离提取 工艺优化 |
Initial Screening of High Glutathione-producing Strain and Optimization of Extraction of Reduced-glutathione |
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| WANG Shuang,ZHANG Hui-wen,YE Shu-hong,XU Ming-kai.Initial Screening of High Glutathione-producing Strain and Optimization of Extraction of Reduced-glutathione[J].Biotechnology,2011,21(3). |
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| Authors: | WANG Shuang ZHANG Hui-wen YE Shu-hong XU Ming-kai |
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| Institution: | WANG Shuang1,ZHANG Hui-wen2,YE Shu-hong1,*,XU Ming-kai2,*(1.College of Food Science,Dalian Polytechnic University,Dalian 116034,China,2.Institute of Applied Ecology,Chinese Academy of Sciences,Shenyang 110016,China) |
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| Abstract: | Objective: To screen a high-yield strains of the reduced glutathione(GSH) and optimized the extraction technology.Method: Selected the high-yield yeast of GSH from the IAE culture preservation library,adjusted the media component to increase the GSH production,and optimized the extraction conditions in two methods-hot water and ethanol extraction to improve the GSH content.Result: A GSH high-yield Saccharomyces cerevisiae Y,with the production of 9.6 mg GSH per gram dry cells,was obtained.After adjusting of... |
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| Keywords: | reduced-glutathione(GSH) Saccharomyces cerevisiae medium component separation and extraction technology optimization |
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