| 中度嗜盐菌LY9的分离鉴定及其淀粉酶特性研究 |
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| 引用本文： | 李新,孙晓,李悦佳,高霞,杜崇旭.中度嗜盐菌LY9的分离鉴定及其淀粉酶特性研究[J].生物技术,2011,21(3). |
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| 作者姓名： | 李新 孙晓 李悦佳 高霞 杜崇旭 |
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| 作者单位： | 1. 运城学院生命科学系,山西运城,044000
2. 大连民族学院,大连辽宁,116600 |
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| 基金项目： | 运城学院博士启动基金项目 |
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| 摘 要： | 目的:从运城盐湖中分离获得中度嗜盐菌,并对其进行分离鉴定及酶学特性研究.方法:采用淀粉底物平板法获得高产胞外淀粉酶的嗜盐菌株LY9.16S rRNA序列分析,结合形态学和生理生化特征分析对其进行分类鉴定.研究不同物理化学因素对淀粉酶活性的影响.结果:系统发育分析表明该菌为Halobacillus属成员,鉴定并命名为Halobacillus sp.LY9,为中度嗜盐菌.该淀粉酶最适作用温度和pH值分别为60℃和8.0,同时在较宽pH范围内(4.0～12.0)保持高活力,表现出了较强的抗酸碱能力.Cu2+可明显抑制该酶活性,其它金属离子则基本无影响;该酶不受EDTA的抑制,表明该酶不属于金属蛋白酶,但SDS却能使酶活明显降低.结论:LY9的分离鉴定及其淀粉酶特性研究对促进运城盐湖生物资源的开发利用具有重要意义.
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| 关 键 词： | 中度嗜盐菌 淀粉酶 分离鉴定 酶学特性 |
Characterization of a Moderate Halophole LY9 and Studies on the Properties of Its Extracellular Amylase |
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| LI Xin,SUN Xiao,LI Yue-jia,GAO Xia,DU Chong-xu.Characterization of a Moderate Halophole LY9 and Studies on the Properties of Its Extracellular Amylase[J].Biotechnology,2011,21(3). |
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| Authors: | LI Xin SUN Xiao LI Yue-jia GAO Xia DU Chong-xu |
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| Institution: | LI Xin1,SUN Xiao1,LI Yue-jia1,GAO Xia1,DU Chong-xu2*(1.College of Life Sciences,Yuncheng University,Yuncheng 044000,China,2.Dalian Nationalities University,Dalian 116600,China) |
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| Abstract: | Objective: A moderately halophilic bacterium was isolated from salt lake of Yuncheng,and its characterization and amylase properties were studied.Method: Using starch plate method,the strain LY9 producing extracellular amylase was obtained.Morphological tests,biochemical reactions and 16S rRNA sequence analysis were carried to identify the bacteria.Different physicochemical factors on the amylase activity were also studied.Result: The strain was identified as genus of Halobacillus,and named as Halobacillus ... |
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| Keywords: | Halobacillus |
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